CAR-T cells can subsequently be purified and isolated by ProMab Biotechnologies CAR-T Extraction Kit.
Figure 4. Scheme of CAR-T cell isolation using ProMab Biotechnologies' antibody-coated magnetic beads
We have also quantified that the increase of multiplicity of infection (MOI) correlates to an increase in transduction efficiency and CAR expression.
Figure 5. Comparison by flow cytometry of tagged CAR-T cells generated at either a MOI of 5 or 10.