E. coli Expression

ProMab Biotechnologies has years of experience providing CRO services with a skilled team of scientists who will aid you in developing your protein or peptide of interest. We offer protein expression services utilizing e. coli expression, baculovirus expression, mammalian expression, with additional services including gene synthesis and peptide synthesis.

Recombinant protein expression typically begins with designing and producing a plasmid that encodes for the desired protein. Following the construction, the plasmid is introduced into the host cell and scaled-up to induce protein expression. The cells are lysed and the proteins are extracted, purified, and validated using SDS-PAGE and western blotting.

E. coli is widely used as a host cell to produce recombinant proteins with pharmaceutical or industrial targets. E. coli expression systems are easy to purify, cost and time efficient, and have good stability and high expression. However, due to a deficiency of post-translational modification machinery in E. coli, proteins will not be modified unlike in mammalian cells.

E. Coli Expression Service

Our high-level recombinant protein E. coli expression service includes scale-up and tag removal as requested, fast turn-around time, and competitive pricing. E. coli expression is among the most widely used systems for recombinant proteins because of its many advantages.

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Breakdown of Standard Service by Phase

Our E. coli expression service takes 6 to 8 weeks from subcloning to expression in 2 phases starting at $3,200.

Phase I	Phase II
Subcloning into Expression Vector Amplification/isolation of the gene of interest (GOI) from a client-supplied construct and subcloning into a suitable transfer vector with His-Tag (or GST-tag). Sequence confirmation of insert. Mini-induction to over-express the target protein. Test for recombinant protein by SDS-PAGE and Western Blot. The customer must provide appropriate antibodies to the protein of interest if desired.	Expansion and Purification One liter of bacterial culture will be induced with IPTG and harvested for protein purification. Ni-NTA purification of protein with 6X His-tags or glutathione for GST-tagged proteins using beads either under native or denatured conditions.
Estimated time: 3-5 weeks Price: $2,000	Estimated time: 2 weeks Price: $1,200
Deliverables 5 μg of transfer vector Recombinant Protein Documentation Yield ranges from 0.5 – 50 mg/L of culture with an average yield of 3 mg/L. Solubility is not guaranteed.