To decrease the immunogenicity of monoclonal antibodies derived from rodents, we use a rational approach based on antibody sequence and 3D structure by computer modeling and docking programs. In brief, steps include: hybridoma sequencing, analysis of the scFv by different bioinformatics and computer programs, generating best models and testing these antibodies efficacy.
Alongside ProMab's technology platform, we can ultimately retest these humanized scFvs in a CAR-T/NK setting using various in vitro or in vivo studies.
Figure 2. An illustration of the stepwise process of humanizing a rodent mAb for generating a CAR-T cell with a humanized scFv.
This is done by a group of bioinformaticists and computer modeling professionals with many years of experience in antibody humanization with references (1-3) below.
- Alexey Teplyakov, Galina Obmolova, Thomas J. Malia, Jinquan Luo, Salman Muzammil, Raymond Sweet, Juan Carlos Almagro & Gary L. Gilliland. Structural diversity in a human antibody germline library. mAbs. V9, 2016, 1045-63.
- Almagro J.C., Teplyakov A., Luo J., Sweet R.W, Kodangattil S, hernandez-Guzman F, Gilliand G.L. Second antibody modeling assessment (AMA-II). Proteins 2014; 82: 1553-1562.
- Almagro JC, Fransson J. Humanization of antibodies. Front Biosci., 2008, 1;13:1619-33.