Custom CAR-NK Manufacturing
Accelerate your novel cancer immunotherapy development by leveraging our cutting-edge CAR-NK manufacturing platform. As the most experienced CRO in the CAR-T cell industry, having developed over 60 types of CAR-T/NK cells and target cells in the last 5 years, we provide a complete and reliable service to drive your project forward.
- Generate large numbers of cells with high purity.
- Benefit from several patented innovations in CAR construct design.
- Choose from mRNA electroporation or lentivirus transduction.
- Supply your own monoclonal antibodies or leave development to us.
Choose ProMab for your CAR-NK manufacturing
With their efficient tumor-killing power, CAR-NK cells are a vital tool in the development of novel immunotherapies. We understand that you need access to quality cells-fast-when tackling cancers such as multiple myeloma, leukemia, and sarcoma. That’s why we developed our cutting-edge platform to generate large numbers of cells with high purity, arming you with the tools you need for your research.
Our platform, your cells
At ProMab, you can use our platform to generate customized cells to your own specifications. Our platform has 10 core components that can be configured for your own personalized needs for CAR-NK manufacturing.
We can begin the process with a plan to include monoclonal antibody development or base the plan on your own existing antibody sequence to generate CAR constructs, giving you full control. Once the CAR constructs have been generated, you can choose between mRNA electroporation or lentivirus transduction as a delivery system to create your cells. To ensure that your cells are of the highest purity and quality, they undergo a range of functional assays and tuning, with continued optimization until they meet your project needs.
CAR-NK workflow showing lentivirus transduction.
| CAR-NK PLATFORM | ANTIBODY SCREENING |
HYBRIDOMA SEQUENCING |
mRNA TRANSFECTION |
LENTIVIRUS PRODUCTION |
|
CAR-NK CELL PRODUCTION |
RTCA & CYTOKINE ASSAY |
AFFINITY TUNE-UP & HUMANIZATION |
ANIMAL STUDIES |
CAR-NK CELL EXPANSION & OPTIMIZATION |
CAR construct plasmid
Streamlining Your Preclinical and Clinical Development
Using our experience in CAR research, we are able to offer preclinical and clinical development studies as part of our CAR-NK service. Making use of our in-house vivarium and 2 GMP manufacturing facilities, we can take care of your CAR-NK discovery and development needs, whatever your project.
We offer a development service that is tailored to you and your project, including options for licensing and partnering. Contact us to find out how we can streamline your preclinical and clinical development today.
Discovery Services Timelines
Accelerate your research timelines by making use of our comprehensive discovery services. We provide in vitro and in vivo offerings all within a single organization, making the process as simple as possible for you. With our end-to-end solution, you can tailor our discovery and development services to suit you, whatever your starting point.
Develop Antibodies
Create mouse, rabbit, and human monoclonal antibodies using our validated antibody design and development platforms.
Package Vectors
Innovate your cancer immunotherapies by packaging novel co-expression constructs and any generation of CAR domain into your vectors.
Scale Production
Expand your research horizons with scalable CAR-NK cell production from our GMP manufacturing facilities.
Ensure Quality
Get reliable CAR expression, cytokine release, and cytotoxicity measurements from our expert team with years of CAR immune cell experience.
Quality you can rely on
NK cell purity is >80% on day 9 and >95% on day 14 of culture.
NK cell numbers expand 35-fold on day 9 and 75-fold on day 14.
Human PBMC were transduced with lentivirus encoding the CAR; seven days later, gated NK cells were analyzed by flow cytometry for CAR expression.
NK cells were generated from human PBMC, electroporated with CAR mRNA, and analyzed by flow cytometry for CAR expression 24 hours later.
Service plans and prices
To allow for either custom antibody creation or the use of your own antibody, we offer the following service plans and prices for CAR-NK manufacturing:
Plan A
Construct a CAR based on your antibody sequence construct from a mouse monoclonal antibody or fully human single-chain variable antibody fragment (scFv) from our existing library.
| DEVELOPMENT STAGE | DESCRIPTION | Price |
|---|---|---|
| Phase I (optional) | Immunization of Balb/C mice with recombinant protein or carrier-conjugated peptide. Generation of hybridoma cell lines following antibody validation against the correctly presented antigen. | $6,995 |
| Phase II (optional) | Sequencing of the antibody's heavy and light chains. | $1,800 |
| Phase III | Synthesis and cloning of an scFv with selected CAR domains into a lentivirus vector. Generation of 1x108 lentivirus viral particles. | $7,000 |
| Phase IV | Transduction with lentivirus or electroporation with the CAR mRNA. FACS analysis to validate CAR expression in NK cells. | $6,000 to 8,000 |
| Phase V | Measurement of CAR function: real-time cytotoxicity assay, quantitation of cytokine production (IFN-γ, IL-2, Granzyme B, GM-CSF). | $7,000 |
Plan B
Generate the entire construct from a mouse monoclonal antibody or fully human single-chain variable antibody fragment (scFv) from our existing library.
| DEVELOPMENT STAGE | DESCRIPTION | Price |
|---|---|---|
| Phase III |
Synthesis and cloning of an scFv with selected CAR domains into a lentivirus vector. Generation of 1x10^8 lentivirus viral particles. |
$6,000 |
| Phase IV | Transduction with lentivirus or electroporation with the CAR mRNA. FACS analysis to validate CAR expression in NK cells. | $6,000 to $8,000 |
| Phase V | Measurement of CAR function: real-time cytotoxicity assay, quantitation of cytokine production (IFN-γ, IL-2, Granzyme B, GM-CSF). | $8,000 |
| Phase V | Tumor study in NSG/NOG mice. | Starting at $15,000 |
Start manufacturing your cells today
We’ll take care of your CAR-NK manufacturing so that you can focus on insights and innovations. Together, we can change the way the world treats cancer.
5,000+
monoclonal antibodies generated
50+
filed patents
500+
CAR-T projects finished
4
Phase 2 clinical trials started
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FAQs
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