ProMab Biotechnologies has developed a growing list of engineered cell lines including CAR-T/NK cells and target cells, i.e. GFP-Raji cells.
In nearly 5 years we have developed over 30 types of CAR-T/NK cells and target cells with several patented innovations in the CAR construct design. We can construct a CAR based on your antibody sequence or we can generate the entire construct from a mouse monoclonal antibody or fully human single-chain variable antibody fragment (scFv) from our existing library.
Our CAR-T platform will work very similarly to developing CAR-NK cells, with some subtle differences in project design.
We have plans that include monoclonal antibody development or are based on using your existing antibody sequence to generate the CAR constructs. When client requires antibody developed then Plan A is available starting at $22,500. When client provides antibody sequence Plan B starts at $16,950.
Plan A - Client receives 2 clones of hybridoma cell lines along with 2 x 108 CAR-NK cells cryopreserved.
Plan B - Client receives 2 x 108 CAR-NK cells cryopreserved.
Service Plans and Prices
PLAN A
| DEVELOPMENT STAGE | DESCRIPTION | Price |
|---|---|---|
| Phase I (optional) | Immunization of Balb/C mice with recombinant protein or carrier-conjugated peptide. Generation of hybridoma cell lines following antibody validation against the correctly-presented antigen. | $5,150 |
| Phase II (optional) | Sequencing of the antibody's heavy and light chains. | $1,600 |
| Phase III | Synthesis and cloning of an scFv with selected CAR domains into a lentivirus vector. Generation of 1x108 lentivirus viral particles. | $6,000 |
| Phase IV | Lentiviral transduction, FACS analysis to validate CAR expression in NK cells. | $5,000 |
| Phase V | Measurements of CAR expression, cytokines (such as IFN-γ, IL-2, GranzymeB) and cytotoxicity via RTCA. | $6,000 |
PLAN B
| DEVELOPMENT STAGE | DESCRIPTION | PRICE |
|---|---|---|
| Phase III | Synthesis and cloning of an scFv with selected CAR domains into a lentivirus vector. Generation of 1x108 lentivirus viral particles. | $6,000 |
| Phase IV | Lentiviral transduction, FACS analysis to validate CAR expression in NK cells. | $5,000 |
| Phase VI | NSG/NOG xenograft model | starting at $15,000 |
We also have off the shelf solutions that will quickly establish and advance your projects in the following areas: