We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application.
Your CAR-T scFv Could Be Generated From One Of Our Three Antibody Production Platforms:
To decrease the immunogenicity of monoclonal antibodies derived from rodents, ProMab Biotechnologies uses a rational approach based on antibody sequence and 3D structure by computer modeling and docking programs. In brief, steps include: hybridoma sequencing, analysis of the scFv by different bioinformatics and computer programs, generating best models and retesting humanized scFvs in a CAR-T setting.
We provide comprehensive tools for scFv validation in vitro. The scFv validation platform contains an antigen/cell binding assay, a cell killing assay, and a cytokine induction assay. Our tools include 1) 42 DNA fingerprint-certified authenticated cell lines; 2) 8,000 tissue blocks - 5000 from diseased tissues, 3000 from normal tissues; 3) >30 types of primary cells. All of these can be used for antigen expression profiling to improve the CAR-T therapeutic index.
Tissue Array For IHC Staining
This is done by a group of bioinformaticists and antibody engineering experts with many years of experience in antibody humanization with references below.
- Alexey Teplyakov, Galina Obmolova, Thomas J. Malia, Jinquan Luo, Salman Muzammil, Raymond Sweet, Juan Carlos Almagro & Gary L. Gilliland. Structural diversity in a human antibody germline library. mAbs. V9, 2016, 1045-63.
- Almagro J.C., Teplyakov A., Luo J., Sweet R.W, Kodangattil S, hernandez-Guzman F, Gilliand G.L. Second antibody modeling assessment (AMA-II). Proteins 2014; 82: 1553-1562.
- Almagro JC, Fransson J. Humanization of antibodies. Front Biosci., 2008, 1;13:1619-33.
Other Components of the CAR Platform: