Promab Biotechnologies has generated different CAR-T/CAR-NK products such as: CAR-T cells are T cells either from the group of donors or from individual donor transduced with CAR (Chimeric antigen receptor)-lentivirus. The CAR-T cells can target different tumor antigens such CD19, CD133, Her-2, EGFR, VEGFR-2, mesothelin that are highly over-expressed in tumors. The CAR-T cells can express inducible caspase-9 or other suicide gene to induce CAR-T cell killing for increased safety in case this construct in future will be proposed to use in clinical trials. All CAR-T cells are checked for transduction efficiency (Fab Ab by Flow cytometry), cell killing activity: IL-2, IFN-gamma assay; and Real time cytotoxicity activity). All CAR-T cells have negative mock controls: lentivirus-transduced CAR-T cells, no ScFv-transduced CAR-T cells and non transduced T cells.
Promab Biotechnologies has generated the following CAR-T products:
CAR-T Cells
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR1000-1M |
'Mock' ScFv Control |
1x10 6 /vial |
$1,000 |
 |
| PM-CAR1001-1M |
CD19scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1002-1M |
CD19scFv-4-1BB-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1003-1M |
CD19scFv-CD28-4-1BB-CD3Ī¶ |
1x10 6 /vial |
$800 |
|
| PM-CAR1004-1M |
CD19scFv-CD28-4-1BB |
1x10 6 /vial |
$800 |
|
| PM-CAR1006-1M |
iCas9-T2A-antiCD19scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,200 |
|
| PM-CAR1007-1M |
CD19scFv-FLAG-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1008-1M |
no scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,200 |
|
| PM-CAR1009-1M |
CD19scFv-CD28 |
1x10 6 /vial |
$800 |
|
| PM-CAR1010-1M |
iCas9 HA-T2A-antiCD19scFv-CD28-CD3Ī¶āGGGS-FLAG |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1011-1M |
Mesothelin scFv-CD28-CD3Ī¶ (2nd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1012-1M |
Mesothelin scFv-CD28-4-1BB-CD3Ī¶ (3rd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1013-1M |
Mesothelin scFv-4-1BB-CD3Ī¶ (2nd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1014-1M |
Mesothelin scFv-FLAG-CD28-4-1BB-CD3Ī¶ (3rd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1015-1M |
Mesothelin scFv-FLAG-4-1BB-CD3Ī¶ (2nd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1016-1M |
VEGFR2scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1017-1M |
GPC3 scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1018-1M |
CD133 scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1019-1M |
EpCAM1 scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1020-1M |
EpCAM*scFv-CD28-CD3Ī¶ * NheI restriction site introduced, N-terminal of scFV (amino acids: AS) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1021-1M |
EGFRscFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1022-1M |
EGFRscFv-4-1BB-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1023-1M |
EGFRscFv-GITR-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1024-1M |
Her2 scFv-CD28-CD3Ī¶ |
1x10 6 /vial |
$1,000 |
|
Ā Call us for details.
Non-Transduced T Cells
The non-transduced T cells are available in frozen vials that can be activated and expanded with CD28/CD3 beads and used as a negative control in all assays with CAR-T cells. Promab Biotechnologies has non transduced T cells from different donor that can be used in the assay to test individual differences among different donors.
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR2003 |
Non-transduced T-cells, healthy donor |
1x10 6 /vial |
$500 |
|
| PM-CAR2004 |
Non-transduced T-cells, ALL |
1x106/vial |
$500 |
|
| PM-CAR2005 |
Non-transduced T-cells, CLL |
1x106/vial |
$500 |
|
| PM-CAR2006 |
Non-transduced T-cells, NHL |
1x106/vial |
$500 |
|
Engineered CAR-T Target Cells
Promab Biotechnologies has developed several target cell lines to be used in cytotoxicity assays with CAR-T effector cells. These cells express tumor antigen that is overexpressed in malignant cells, can have GFP or luciferase for in vivo studies.
| Catalog # |
Description |
Marker |
Cell Number |
Price |
Datasheet |
| PM-Raji-GFP |
Raji-GFP stable expression |
CD19+, eGFP |
1x106/vial |
$6,000 |
|
| PM-Jurkat-GFP |
Jurkat-GFP stable expression |
CD19-, eGFP |
1x106/vial |
$6,000 |
|
| PM-K562-GFP |
K562-GFP stable expression |
CD19-, eGFP |
1x106/vial |
$6,000 |
|
| PM-HELA-CD19 |
HeLa-CD19 stable expression |
CD19+ |
1x106/vial |
$8,000 |
|
| PM-CHO-CD19 |
CHO-CD19 stable expression |
CD19+ |
1x106/vial |
$6,000 |
|
PBMC
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR2007 |
PBMC, ALL |
1x106/vial |
$500 |
|
| PM-CAR2008 |
PBMC-CLL |
1x106/vial |
$500 |
|
| PM-CAR2009 |
PBMC-NHL |
1x106/vial |
$500 |
|
Cell Media and Activation Beads
Under Current Development
| Her-3(Erb-3) |
Her-4(Erb-4) |
CEA |
EGFRviii |
PSMA |
CD20 |
| BCMA |
VEGFR-1 |
VEGFR-3 |
CD22 |
c-Met |
Lewis A |
| ROR-1 |
CD326 |
CD133 |
|
|
|
