Car-T Cells for Research

Promab Biotechnologies has generated CARs (chimeric antigen receptors) specific for a variety of target antigens, many of which are overexpressed on tumor cells. These target antigens include CD19, CD22, CD133, Her-2, EGFR, Mesothelin, and more (see Table, below). In addition, Promab has modified the CARs in a number of ways to increase their utility, including adding inducible caspase-9 as a safety switch; adding non-immunogenic tags to provide for CAR identification and selection; and using different costimulatory domains to modulate CAR functional responses.


Promab Biotechnologies uses high-titer lentivirus transduction to introduce the CARs into T cells, generating CAR-T cells. Promab has also generated CAR-NK cells and CAR-macrophages for a subset of CARs. The CAR-T cells are validated using multiple methods, including flow cytometry (to determine the CAR-T frequency and phenotype), real-time and end-point cytotoxicity assays, cytokine production and checkpoint protein upregulation.


For a negative control, Promab has generated a mock CAR which lacks the target-recognizing scFv domain but can be detected with the Beam-2 antibody. Promab also offers non-transduced T cells as another negative control.


Promab Biotechnologies has generated the following CAR-T products:
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Catalog #Target AntigenTagCostimulatory DomainCAR ConstructData Sheet

Catalog # Description Cell Number Price DataSheet
PM-CARMA01 CD19 scFv-Flag CAR THP-1 Cells 1x10/vial $6,000 pdf icon
PM-CARMA02 Mesothelin scFv CAR THP-1 Cells 1x106/vial $6,000 pdf icon

The non-transduced T cells are available in frozen vials that can be activated and expanded with CD28/CD3 beads and used as a negative control in all assays with CAR-T cells. Promab Biotechnologies has non transduced T cells from different donor that can be used in the assay to test individual differences among different donors.

Catalog # Description Cell Number Price DataSheet
PM-CAR2003 Non-transduced T cells, healthy donor 1x10/vial $550 pdf icon
PM-CAR2004 Non-transduced T cells, ALL 1x106/vial $550 pdf icon
PM-CAR2005 Non-transduced T cells, CLL 1x106/vial $550 pdf icon
PM-CAR2006 Non-transduced T cells, NHL 1x106/vial $550 pdf icon
PM-CAR2007 Non-transduced T cells CD4T cells 1x106/vial $1,200 pdf icon
PM-CAR2008 Non-transduced T cells CD8T cells 1x106/vial $1,200 pdf icon
PM-CAR2009 Non-transduced T CD4Tcm cells  1x106/vial $1,200 pdf icon
PM-CAR2010 Non-transduced CD4Tem cells   1x106/vial $1,200 pdf icon
PM-CAR2011 Non-transduced Tnaïve cells  1x106/vial $1,200 pdf icon
PM-CAR2012 Non-transduced Tcm cells 1x106/vial $1,200 pdf icon
PM-CAR2014 Trasnduced GFP+ T cells 1x106/vial $1,200 pdf icon
Catalog # Description Cell Number Price DataSheet
PM-CAR2013 Non-transduced Macrophage Cells 1x106/vial $550 pdf icon

ProMab has developed several target cell lines to be used in cytotoxicity assays with CAR-T effector cells. These cells express specific tumor antigens that are overexpressed in malignant cells, or can have GFP or luciferase for in vivo studies, or knockout target antigens by CRISPR.

Catalog # Description Marker Cell Number Price Datasheet
PM-Raji-GFP Raji-GFP-LUC stable expression GFP+, LUC+, CD19+ 1x106/vial $6,000 pdf icon
PM-Jurkat-GFP Jurkat-GFP-LUC stable expression GFP+, LUC+, CD19 1x106/vial $6,000 pdf icon
PM-K562-GFP K562-GFP-LUC stable expression GFP+, LUC+, CD19 1x106/vial $6,000 pdf icon
PM-BxPC3-GFP-LUC BxPC3-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-RPMI8226-GFP-LUC RPMI8226-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-H929-GFP-LUC H929-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-SKOV-3-GFP-LUC SKOV3-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-CD19-HELA CD19-HeLa stable expression CD19+ 1x106/vial $6,000 pdf icon
PM-BCMA-HELA-GFP BCMA-HeLa-GFP stable expression GFP+ 1x106/vial $6,000 pdf icon
PM-BCMA-CHO BCMA-CHO stable expression BCMA+ 1x106/vial $8,000 pdf icon
PM-CS1-HELA-GFP CS1-HeLa-GFP stable expression GFP+ 1x106/vial $6,000 pdf icon
PM-CD19-CHO CD19-CHO stable expression CD19+ 1x106/vial $8,000 pdf icon
PM-CD22-CHO CD22-CHO stable expression CD22+ 1x106/vial $8,000 pdf icon
PM-CD37-CHO CD37-CHO stable expression CD37+ 1x106/vial $8,000 pdf icon
PM-CD47-CHO CD47-CHO stable expression CD47+ 1x106/vial $8,000 pdf icon
PM-CS1-CHO CS1-CHO stable expression CS1+ 1x106/vial $8,000 pdf icon
PM-BCMA-K562-GFP BCMA-K562-GFP stable expression GFP+ 1x106/vial $6,000 pdf icon
PM-SKOV3-ROR1-KO ROR1 knockout SKOV3 cell line ROR1- 1x106/vial $6,000 pdf icon
PM-HeLa-ROR1-KO ROR1 knockout HeLa cell line ROR1- 1x106/vial $6,000 pdf icon
Catalog # Description Volume/Unit Price DataSheet
PM-CAR2000 CAR-T Cell Medium (Human Serum) 500 ML $999 pdf icon
PM-CAR2001 CAR-T Cell Medium (Fetal Bovine Serum) 500 ML $659 pdf icon
PM-CAR2002-2ML CD28/CD3 PM-T-Cell Activation/Expansion Beads 2 ML $599 pdf icon
PM-CAR2002-10ML CD28/CD3 PM-T-Cell Activation/Expansion Beads 10 ML $1,900 pdf icon
Her-3(Erb-3) Her-4(Erb-4) CEA EGFRviii PSMA CD20
VEGFR-1 VEGFR-3 c-Met Lewis A ROR-1 CD326
CD133 CD19 CAR+PD-1 siRNA CD19+ TIGIT siRNA BCMA-CS1 BCMA-CD33 NKG2d
MUC1 PSCA PSA CA-125 (MUC16) Notch FLT-3
Catalog # Description Unit Size Price DataSheet
PM-AB001 Beam1 Antibody 100ul, 1 vial $449 pdf icon
PM-AB002 Beam2 Antibody 100ul, 1 vial $449 pdf icon