Promab Biotechnologies has generated CARs (chimeric antigen receptors) specific for a variety of target antigens, many of which are overexpressed on tumor cells. These target antigens include CD19, CD22, CD133, Her-2, EGFR and Mesothelin (see Table, below). In addition, Promab has modified the CARs in a number of ways to increase their utility, including adding inducible caspase-9 as a safety switch; adding non-immunogenic tags to provide for CAR identification and selection; and using different costimulatory domains to modulate CAR functional responses.
Promab Biotechnologies has used high-titer lentivirus transduction to introduce the CARs into T cells, generating CAR-T cells. Promab has also generated CAR-NK cells and CAR-macrophages for a subset of CARs. The CAR-T cells are validated using multiple methods, including flow cytometry (to determine the CAR-T frequency and phenotype), real-time and end-point cytotoxicity assays, cytokine production and checkpoint protein upregulation.
For a negative control, Promab has generated a mock CAR which lacks the target-recognizing scFv domain but can be detected with the Beam-2 antibody. Promab also offers non-transduced T cells as another negative control.
Promab Biotechnologies has generated the following CAR-T products:
CAR-T Cells
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR1000-1M |
Mock scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
 |
| PM-CAR1001-1M |
CD19 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1002-1M |
CD19 scFv-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1003-1M |
CD19 scFv-CD28-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1004-1M |
CD19 scFv-CD28-4-1BB |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1005-1M |
Coming Soon |
|
|
Coming Soon |
| PM-CAR1006-1M |
iCas9-T2A-antiCD19 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1007-1M |
CD19 scFv-FLAG-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1008-1M |
no scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1009-1M |
CD19 scFv-CD28 |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1010-1M |
iCas9 HA-T2A-antiCD19scFv-CD28-CD3ζ−GGGS-FLAG |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1011-1M |
Mesothelin scFv-CD28-CD3ζ (2nd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1012-1M |
Mesothelin scFv-CD28-4-1BB-CD3ζ (3rd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1013-1M |
Mesothelin scFv-4-1BB-CD3ζ (2nd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1014-1M |
Mesothelin scFv-4-1BB-CD3ζ-FLAG |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1015-1M |
Mesothelin scFv-FLAG-4-1BB-CD3ζ (2nd) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1016-1M |
VEGFR2 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1017-1M |
GPC3 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1018-1M |
CD133 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1019-1M |
EpCAM1 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1020-1M |
EpCAM scFv-CD28-CD3ζ * NheI restriction site introduced, N-terminal of scFV (amino acids: AS) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1021-1M |
EGFR scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1022-1M |
EGFR scFv-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1023-1M |
EGFR scFv-TM28-GITR-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1024-1M |
Her2 scFv-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1025-1M |
EGFR scFv-TM28-CD3ζ-GITR |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1026-1M |
CD33 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1027-1M |
CD38 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1028-1M |
CD138 scFv-Beam1-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1029-1M |
CD22 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1030-1M |
CD22 scFv-TM8-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1031-1M |
BCMA-4-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1032-1M |
CD4 scFv-Beam1-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1033-1M |
Humanized BCMA-2 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1034-1M |
Mouse ROR-1 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1035-1M |
CD19 scFv-CD22 scFv-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1036-1M |
Humanized CEA scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1037-1M |
BCMA-4 scFv-TM8-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1038-1M |
NGFR scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1039-1M |
MCAM scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1040-1M |
CD47 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1041-1M |
Humanized CD47 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1042-1M |
Humanized CD19 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1043-1M |
Humanized CD19 scFv-Beam1-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1044-1M |
Human ROR1 scFv-TM28-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1045-1M |
PDL-1 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1046-1M |
CD123 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1047-1M |
CD22 scFv-Beam2-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1048-1M |
CD19 scFv-Beam2-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1049-1M |
CD19 scFv-CD22 scFv-4-1BB-CD3-T2A-tEGFR (Suicide switch) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1050-1M |
CD19 scFv-CD22 scFv-4-1BB-CD3-T2A-RQR8 (Ritiximab suicide switch) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1051-1M |
BCMA-2 scFv-TM-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1052-1M |
CD37 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1053-1M |
CD37 scFv-TM28-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1054-1M |
CD19 scFv-TM28-GITR-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1055-1M |
CD19 scFv-TM8-GITR-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1056-1M |
CD33 scFv-Beam 2-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1057-1M |
CS1 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1058-1M |
B7H4 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1059-1M |
CD24 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1060-1M |
Mesothelin scFv-TM28-CD28-4-1BB-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1061-1M |
3x Beam2-TM28-CD28-CD3ζ (Mock) |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1062-1M |
CD20 scFv-TM28-CD28-CD3ζ |
1x10 6 /vial |
$1,000 |
|
| PM-CAR1063-1M |
Her2 scFv-4-1BB-CD3Z-EGFRt |
1x10 6 /vial |
$1000 |
|
| PM-CAR1064-1M |
Her2 scFv-4-1BB-CD3Z-GFP |
1x10 6 /vial |
$1,000 |
|
CAR Macrophage Cells
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CARMA01 |
CD19 scFv-Flag-CAR THP-1 Cells |
1x106 /vial |
$1000 |
|
| PM-CARMA02 |
Mesothelin scFv-CAR THP-1 Cells |
1x106/vial |
$1,000 |
|
Non-Transduced T Cells
The non-transduced T cells are available in frozen vials that can be activated and expanded with CD28/CD3 beads and used as a negative control in all assays with CAR-T cells. Promab Biotechnologies has non transduced T cells from different donor that can be used in the assay to test individual differences among different donors.
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR2003 |
Non-transduced T cells, healthy donor |
1x106 /vial |
$500 |
|
| PM-CAR2004 |
Non-transduced T cells, ALL |
1x106/vial |
$500 |
|
| PM-CAR2005 |
Non-transduced T cells, CLL |
1x106/vial |
$500 |
|
| PM-CAR2006 |
Non-transduced T cells, NHL |
1x106/vial |
$500 |
|
| PM-CAR2007 |
Non-transduced T cells CD4+ T cells |
1x106/vial |
$1,000 |
|
| PM-CAR2008 |
Non-transduced T cells CD8+ T cells |
1x106/vial |
$1,000 |
|
| PM-CAR2009 |
Non-transduced T CD4+ Tcm cells |
1x106/vial |
$1,000 |
|
| PM-CAR2010 |
Non-transduced CD4+ Tem cells |
1x106/vial |
$1,000 |
|
| PM-CAR2011 |
Non-transduced Tnaïve cells |
1x106/vial |
$1,000 |
|
| PM-CAR2012 |
Non-transduced Tcm cells |
1x106/vial |
$1,000 |
|
| PM-CAR2014 |
Non-transduced T cells, GFP+ |
1x106/vial |
$1,000 |
|
Non-Transduced Macrophages
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR2013 |
Non-transduced Macrophage Cells |
1x106/vial |
$500 |
|
Engineered CAR-T Target Cells
Promab Biotechnologies has developed several target cell lines to be used in cytotoxicity assays with CAR-T effector cells. These cells express tumor antigen that is overexpressed in malignant cells, can have GFP or luciferase for in vivo studies, or knockout cell lines by CRISPR.
| Catalog # |
Description |
Marker |
Cell Number |
Price |
Datasheet |
| PM-Raji-GFP |
Raji-GFP stable expression |
GFP+, CD19+ |
1x106/vial |
$6,000 |
|
| PM-Jurkat-GFP |
Jurkat-GFP stable expression |
GFP+, CD19- |
1x106/vial |
$6,000 |
|
| PM-K562-GFP |
K562-GFP stable expression |
GFP+, CD19- |
1x106/vial |
$6,000 |
|
| PM-CD19-HELA |
CD19-HeLa stable expression |
CD19+ |
1x106/vial |
$6,000 |
|
| PM-CD19-CHO |
CD19-CHO stable expression |
CD19+ |
1x106/vial |
$8,000 |
|
| PM-CD47-CHO |
CD47-CHO stable expression |
CD47+ |
1x106/vial |
$8,000 |
|
| PM-BxPC3-GFP-LUC |
BxPC3-GFP-LUC stable expression |
GFP+, LUC+ |
1x106/vial |
$6,000 |
|
| PM-BCMA-CHO |
BCMA-CHO stable expression |
BCMA+ |
1x106/vial |
$8,000 |
|
| PM-CD22-CHO |
CD22-CHO stable expression |
CD22+ |
1x106/vial |
$8,000 |
|
| PM-CS1-CHO |
CS1-CHO stable expression |
CS1+ |
1x106/vial |
$8,000 |
|
| PM-RPMI8226-GFP-LUC |
RPMI8226-GFP-LUC stable expression |
GFP+, LUC+ |
1x106/vial |
$6,000 |
|
| PM-H929-GFP-LUC |
H929-GFP-LUC stable expression |
GFP+, LUC+ |
1x106/vial |
$6,000 |
|
| PM-SKOV-3-GFP-LUC |
SKOV3-GFP-LUC stable expression |
GFP+, LUC+ |
1x106/vial |
$6,000 |
|
| PM-CD37-CHO |
CD37-CHO stable expression |
CD37+ |
1x106/vial |
$8,000 |
|
| PM-CS1-HELA-GFP |
CS1-HeLa-GFP stable expression |
GFP+ |
1x106/vial |
$6,000 |
|
| PM-BCMA-K562-GFP |
BCMA-K562-GFP stable expression
|
GFP+ |
1x106/vial |
$6,000 |
|
| PM-BCMA-HELA-GFP |
BCMA-HeLa-GFP stable expression |
GFP+ |
1x106/vial |
$6,000 |
|
| PM-SKOV3-ROR1-KO |
ROR1 knockout SKOV3 cell line |
ROR1- |
1x106/vial |
$6,000 |
 |
| PM-HeLa-ROR1-KO |
ROR1 knockout HeLa cell line |
ROR1- |
1x106/vial |
$6,000 |
|
PBMC
| Catalog # |
Description |
Cell Number |
Price |
DataSheet |
| PM-CAR3000 |
PBMC, ALL |
1x106/vial |
$500 |
|
| PM-CAR3001 |
PBMC, CLL |
1x106/vial |
$500 |
|
| PM-CAR3002 |
PBMC, NHL |
1x106/vial |
$500 |
|
Cell Media and Activation Beads
Under Current Development
| Her-3(Erb-3) |
Her-4(Erb-4) |
CEA |
EGFRviii |
PSMA |
CD20 |
| VEGFR-1 |
VEGFR-3 |
c-Met |
Lewis A |
ROR-1 |
CD326 |
| CD133 |
CD19 CAR+PD-1 siRNA |
CD19+ TIGIT siRNA |
BCMA-CS1 |
BCMA-CD33 |
NKG2d |
| MUC1 |
PSCA |
PSA |
CA-125 (MUC16) |
Notch
|
FLT-3 |
CAR Detection Probes
| Catalog # |
Description |
Unit |
Price |
DataSheet |
| PM-AB001 |
Beam1 Antibody |
1 vial |
$399 |
|
| PM-AB002 |
Beam2 Antibody |
1 vial |
$399 |
|
| PM-AB003 |
BCMA Biotinylated Protein |
1 vial |
$399 |
|
| PM-AB004 |
Mesothelin Biotinylated Protein |
1 vial |
$399 |
|