CAR-T Cells
Promab Biotechnologies has generated CARs (chimeric antigen receptors) specific for a variety of target antigens, many of which are overexpressed on tumor cells. These target antigens include CD19, CD22, CD133, Her-2, EGFR, Mesothelin, and more (see Table, below). In addition, Promab has modified the CARs in a number of ways to increase their utility, including adding inducible caspase-9 as a safety switch; adding non-immunogenic tags to provide for CAR identification and selection; and using different costimulatory domains to modulate CAR functional responses.
Promab Biotechnologies uses high-titer lentivirus transduction to introduce the CARs into T cells, generating CAR-T cells. Promab has also generated CAR-NK cells and CAR-macrophages for a subset of CARs. The CAR-T cells are validated using multiple methods, including flow cytometry (to determine the CAR-T frequency and phenotype), real-time and end-point cytotoxicity assays, cytokine production and checkpoint protein upregulation.
For a negative control, Promab has generated a mock CAR which lacks the target-recognizing scFv domain but can be detected with the Beam-2 antibody. Promab also offers non-transduced T cells as another negative control.
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Promab Biotechnologies has generated the following CAR-T products:| Catalog # | Target Antigen | Tag | Costimulatory Domain | CAR Construct | Data Sheet |
| PM-CAR1000 | Mock (Control) | – | CD28 | Mock-TM28-CD28-CD3z |  |
| PM-CAR1001 | CD19 | – | CD28 | CD19scFv-TM-CD28-CD3z | |
| PM-CAR1002 | CD19 | – | 4-1BB | CD19scFv-TM-4-1BB-CD3z | |
| PM-CAR1003 | CD19 | – | CD28-4-1BB | CD19scFv-TM-CD28-4-1BB-CD3z | |
| PM-CAR1004 | CD19 | FLAG (GFP) | CD28 | CD19scFv-FLAG-CD28-CD3z /// GFP | |
|
PM-CAR1005 |
Coming soon | – | Coming soon | Coming soon | Coming soon |
| PM-CAR1006 | CD19 | – | CD28 | iCas9-T2A-CD19scFv-TM-CD28-CD3z | |
| PM-CAR1007 | CD19 | FLAG | CD28 | CD19scFv-FLAG-CD28-CD3z | |
| PM-CAR1008 | – | – | CD28 | TM-CD28-CD3z | |
| PM-CAR1009 | CD19 | – | CD28 | CD19scFv-TM-CD28 | |
| PM-CAR1010 | CD19 | – | CD28 | iCas9-HA-T2A-antiCD19scFv-CD28-CD3z−GGGS-FLAG | |
| PM-CAR1011 | Mesothelin | – | CD28 | MSLNscFv-TM-CD28-CD3z | |
| PM-CAR1012 | Mesothelin | – | CD28-4-1BB | MSLNscFv-TM-CD28-4-1BB-CD3z | |
| PM-CAR1013 | Mesothelin | – | 4-1BB | MSLNscFv-TM-4-1BB-CD3z | |
| PM-CAR1014 | Mesothelin | FLAG | 4-1BB | MSLNscFv-FLAG-TM28-4-1BB-CD3z | |
| PM-CAR1015 | Mesothelin | – | 4-1BB | MSLNscFv-FLAG-TM-4-1BB-CD3z-FLAG | |
| PM-CAR1016 | VEGFR2 | – | CD28 | VEGFR2scFv-TM-CD28-CD3z | |
| PM-CAR1017 | GPC3 | – | CD28 | GPC3scFv-TM-CD28-CD3z | |
| PM-CAR1018 | CD133 | – | CD28 | CD133scFv-TM-CD28-CD3z | |
| PM-CAR1019 | EpCAM | – | CD28 | EpCAMscFv-TM-CD28-CD3z | |
| PM-CAR1020 | EpCAM | – | CD28 | EpCAMscFv-TM-CD28-CD3z | |
| PM-CAR1021 | EGFR | – | CD28 | EGFRscFv-TM-CD28-CD3z | |
| PM-CAR1022 | EGFR | – | 4-1BB | EGFRscFv-TM-4-1BB-CD3z | |
| PM-CAR1023 | EGFR | – | GITR | EGFRscFv-TM28-GITR-CD3z | |
| PM-CAR1024 | Her2 | – | CD28 | Her2scFv-TM-CD28-CD3z | |
| PM-CAR1025 | EGFR | – | GITR | EGFRscFv-TM28-CD3z-GITR | |
| PM-CAR1026 | CD33 | – | CD28 | CD33scFv-TM28-CD28-CD3z | |
| PM-CAR1027 | CD38 | – | CD28 | CD38scFv-TM28-CD28-CD3z | |
| PM-CAR1028 | CD138 | FLAG | CD28 | CD138scFv-FLAG-TM28-CD28-CD3z | |
| PM-CAR1029 | CD22 | – | CD28 | CD22scFv-TM28-CD28-CD3z | |
| PM-CAR1030 | CD22 | – | 4-1BB | CD22scFv-TM8-4-1BB-CD3z | |
| PM-CAR1031 | BCMA | – | CD28 | BCMAscFv-TM28-CD28-CD3z | |
| PM-CAR1032 | CD4 | FLAG | CD28 | CD4scFv-FLAG-TM28-CD28-CD3z | |
| PM-CAR1033 | BCMA | – | CD28 | huBCMAscFv-TM28-CD28-CD3z | |
| PM-CAR1034 | ROR1 | – | CD28 | mROR1scFv-TM28-CD28-CD3z | |
| PM-CAR1035 | CD19 + CD22 | – | 4-1BB | CD19scFv-CD22scFv-TM-4-1BB-CD3z | |
| PM-CAR1036 | CEA | – | CD28 | huCEAscFv-TM28-CD28-CD3z | |
| PM-CAR1037 | BCMA | – | 4-1BB | BCMAscFv-TM8-4-1BB-CD3z | |
| PM-CAR1038 | NGFR | – | CD28 | NGFRscFv-TM28-CD28-CD3z | |
| PM-CAR1039 | MCAM | – | CD28 | MCAMscFv-TM28-CD28-CD3z | |
| PM-CAR1040 | CD47 | – | CD28 | CD47scFv-TM28-CD28-CD3z | |
| PM-CAR1041 | CD47 | – | CD28 | huCD47scFv-TM28-CD28-CD3z | |
| PM-CAR1042 | CD19 | – | CD28 | huCD19scFv-TM28-CD28-CD3z | |
| PM-CAR1043 | CD19 | FLAG | CD28 | huCD19scFv-FLAG-TM28-CD28-CD3z | |
| PM-CAR1044 | ROR1 | – | 4-1BB | huROR1scFv-TM28-4-1BB-CD3z | |
| PM-CAR1045 | PDL-1 | – | CD28 | PDL1scFv-TM28-CD28-CD3z | |
| PM-CAR1046 | CD123 | – | CD28 | CD123scFv-TM28-CD28-CD3z | |
| PM-CAR1047 | CD22 | TF | CD28 | CD22scFv-TF-TM28-CD28-CD3z | |
| PM-CAR1048 | CD19 | TF | CD28 | CD19scFv-TF-TM28-CD28-CD3z | |
| PM-CAR1049 | CD19 + CD22 | tEGFR | 4-1BB | CD19scFv-CD22scFv-TM-4-1BB-CD3z-T2A-tEGFR | |
| PM-CAR1050 | CD19 + CD22 | RQR8 | 4-1BB | CD19scFv-CD22scFv-TM-4-1BB-CD3z-T2A-RQR8 | |
| PM-CAR1051 | BCMA | – | CD28 | BCMAscFv-TM-CD28-CD3z | |
| PM-CAR1052 | CD37 | – | CD28 | CD37scFv-TM28-CD28-CD3z | |
| PM-CAR1053 | CD37 | – | 4-1BB | CD37scFv-TM8-4-1BB-CD3z | |
| PM-CAR1054 | CD19 | – | GITR | CD19scFv-TM28-GITR-CD3z | |
| PM-CAR1055 | CD19 | – | GITR | CD19scFv-TM8-GITR-CD3z | |
| PM-CAR1056 | CD33 | TF | CD28 | CD33scFv-TF-TM28-CD28-CD3z | |
| PM-CAR1057 | CS1 | – | CD28 | CS1scFv-TM28-CD28-CD3z | |
| PM-CAR1058 | B7H4 | – | CD28 | B7H4scFv-TM28-CD28-CD3z | |
| PM-CAR1059 | CD24 | – | CD28 | CD24scFv-TM28-CD28-CD3z | |
| PM-CAR1060 | Mesothelin | – | CD28-4-1BB | MSLNscFv-TM28-CD28-4-1BB-CD3z | |
| PM-CAR1061 | Mock (Control) | TF | CD28 | 3xTF-TM28-CD28-CD3z | |
| PM-CAR1062 | CD20 | – | CD28 | CD20scFv-TM28-CD28-CD3z | |
| PM-CAR1063 | Her2 | tEGFR | 4-1BB | Her2scFv-TM-4-1BB-CD3z /// tEGFR | |
| PM-CAR1064 | Her2 | GFP | 4-1BB | Her2scFv-TM-4-1BB-CD3z /// GFP | |
| PM-CAR1065 | c-Met | – | 4-1BB | c-METscFv-TM-4-1BB-CD3z | Coming soon |
| PM-CAR1066 | CD19 | FLAG, tEGFR | CD28 | CD19 scFv-TM-FLAG-CD28-CD3z /// tEGFR |
Coming soon |
| PM-CAR1067 | CD19 | FLAG, tEGFR | 4-1BB | CD19 scFv-TM-FLAG-4-1BB-CD3z /// tEGFR |
Coming soon |
| PM-CAR1068 | EpCAM | – | 4-1BB | EpCAMscFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1069 | PSMA | – | 4-1BB | PSMAscFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1070 | Her2 | – | 4-1BB | Her2scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1071 | CD19 | GFP | 4-1BB | CD19scFv-TM-4-1BB-CD3z-T2A-GFP |
Coming soon |
| PM-CAR1072 | EphA2 | – | 4-1BB | EphA2scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1073 | CD38 | – | 4-1BB | CD38scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1074 | CD70 | – | 4-1BB | CD70scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1075 | CD147 | – | 4-1BB | CD147scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1076 | EpCAM | – | 4-1BB | EpCAMscFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1077 | CLDN 18.2 | – | 4-1BB | CLDN18.2scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1078 | Folate receptor alpha chain | – | 4-1BB | FRalpha scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1079 | alpha Fetaprotein | – | 4-1BB | ⍺FPscFv-TM-4-1BB-CD3ζ |
Coming soon |
| PM-CAR1080 | CD20 | – | 4-1BB | CD20scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1081 | CD22 | – | 4-1BB | CD22scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1082 | Coming soon | – | Coming soon | Coming soon |
Coming soon |
| PM-CAR1083 | Her2 | GFP | CD28 | Her2scFv-TM-CD28-CD3z /// GFP |
Coming soon |
| PM-CAR1084 | Her2 | tEGFR | CD28 | Her2scFv-TM-CD28-CD3z /// tEGFR |
Coming soon |
| PM-CAR1085 | GPC3 | – | 4-1BB | GPC3scFv-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1086 | Mock (Control) | TF | 4-1BB | 3xTF-TM-4-1BB-CD3z |
Coming soon |
| PM-CAR1087 | CD19 | TF | CD28-4-1BB | CD19scFv-TF-CD28-4-1BB-CD3z |
Coming soon |
| PM-CAR1088 | CD19 | FLAG, GFP | CD28-4-1BB | CD19scFv-FLAG-CD28-4-1BB-CD3z /// GFP |
Coming soon |
| PM-CAR1089 | EGFR | GFP | 4-1BB | EGFRscFv-TM-4-1BB-CD3z /// GFP |
Coming soon |
| PM-CAR1090 | CD19 | FLAG, GFP | 4-1BB | CD19 scFv-FLAG-4-1BB-CD3z /// GFP |
Coming soon |
| PM-CAR1091 | PSCA | – | 4-1BB | PSCA scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1092 | CLL-1 | – | 4-1BB | CLL-1 scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1093 | CA-125 | – | 4-1BB | CA-125 scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1094 | GD2 | – | 4-1BB | GD2 scFv-4-1BB-CD3z |
Coming soon |
| PM-CAR1095 | Mucin-1 | – | 4-1BB | Mucin-1 scFv-4-1BB-CD3z |
Coming soon |
CAR Macrophage Cells
| Catalog # | Description | Cell Number | Price | DataSheet |
| PM-CARMA01 | CD19 scFv-Flag CAR THP-1 Cells | 1x106 /vial | $6,000 |  |
| PM-CARMA02 | Mesothelin scFv CAR THP-1 Cells | 1x106/vial | $6,000 | |
Non-Transduced T Cells
The non-transduced T cells are available in frozen vials that can be activated and expanded with CD28/CD3 beads and used as a negative control in all assays with CAR-T cells. Promab Biotechnologies has non transduced T cells from different donor that can be used in the assay to test individual differences among different donors.
| Catalog # | Description | Cell Number | Price | DataSheet |
| PM-CAR2003 | Non-transduced T cells, healthy donor | 1x106 /vial | $550 | |
| PM-CAR2004 | Non-transduced T cells, ALL | 1x106/vial | $550 | |
| PM-CAR2005 | Non-transduced T cells, CLL | 1x106/vial | $550 | |
| PM-CAR2006 | Non-transduced T cells, NHL | 1x106/vial | $550 | |
| PM-CAR2007 | Non-transduced T cells CD4+ T cells | 1x106/vial | $1,200 | |
| PM-CAR2008 | Non-transduced T cells CD8+ T cells | 1x106/vial | $1,200 | |
| PM-CAR2009 | Non-transduced T CD4+ Tcm cells | 1x106/vial | $1,200 | |
| PM-CAR2010 | Non-transduced CD4+ Tem cells | 1x106/vial | $1,200 | |
| PM-CAR2011 | Non-transduced Tnaïve cells | 1x106/vial | $1,200 | |
| PM-CAR2012 | Non-transduced Tcm cells | 1x106/vial | $1,200 | |
| PM-CAR2014 | Trasnduced GFP+ T cells | 1x106/vial | $1,200 | |
Non-Transduced Macrophages
| Catalog # | Description | Cell Number | Price | DataSheet |
| PM-CAR2013 | Non-transduced Macrophage Cells | 1x106/vial | $550 | |
Engineered CAR-T Target Cells
ProMab has developed several target cell lines to be used in cytotoxicity assays with CAR-T effector cells. These cells express specific tumor antigens that are overexpressed in malignant cells, or can have GFP or luciferase for in vivo studies, or knockout target antigens by CRISPR.
| Catalog # | Description | Marker | Cell Number | Price | Datasheet |
| PM-Raji-GFP | Raji-GFP-LUC stable expression | GFP+, LUC+, CD19+ | 1x106/vial | $6,000 | |
| PM-Jurkat-GFP | Jurkat-GFP-LUC stable expression | GFP+, LUC+, CD19- | 1x106/vial | $6,000 | |
| PM-K562-GFP | K562-GFP-LUC stable expression | GFP+, LUC+, CD19- | 1x106/vial | $6,000 | |
| PM-BxPC3-GFP-LUC | BxPC3-GFP-LUC stable expression | GFP+, LUC+ | 1x106/vial | $6,000 | |
| PM-RPMI8226-GFP-LUC | RPMI8226-GFP-LUC stable expression | GFP+, LUC+ | 1x106/vial | $6,000 | |
| PM-H929-GFP-LUC | H929-GFP-LUC stable expression | GFP+, LUC+ | 1x106/vial | $6,000 | |
| PM-SKOV-3-GFP-LUC | SKOV3-GFP-LUC stable expression | GFP+, LUC+ | 1x106/vial | $6,000 | |
| PM-CD19-HELA | CD19-HeLa stable expression | CD19+ | 1x106/vial | $6,000 | |
| PM-BCMA-HELA-GFP | BCMA-HeLa-GFP stable expression | GFP+ | 1x106/vial | $6,000 | |
| PM-BCMA-CHO | BCMA-CHO stable expression | BCMA+ | 1x106/vial | $8,000 | |
| PM-CS1-HELA-GFP | CS1-HeLa-GFP stable expression | GFP+ | 1x106/vial | $6,000 | |
| PM-CD19-CHO | CD19-CHO stable expression | CD19+ | 1x106/vial | $8,000 | |
| PM-CD22-CHO | CD22-CHO stable expression | CD22+ | 1x106/vial | $8,000 | |
| PM-CD37-CHO | CD37-CHO stable expression | CD37+ | 1x106/vial | $8,000 | |
| PM-CD47-CHO | CD47-CHO stable expression | CD47+ | 1x106/vial | $8,000 | |
| PM-CS1-CHO | CS1-CHO stable expression | CS1+ | 1x106/vial | $8,000 | |
| PM-BCMA-K562-GFP | BCMA-K562-GFP stable expression | GFP+ | 1x106/vial | $6,000 | |
| PM-SKOV3-ROR1-KO | ROR1 knockout SKOV3 cell line | ROR1- | 1x106/vial | $6,000 | |
| PM-HeLa-ROR1-KO | ROR1 knockout HeLa cell line | ROR1- | 1x106/vial | $6,000 | |
Cell Media and Activation Beads
| Catalog # | Description | Volume/Unit | Price | DataSheet |
| PM-CAR2000 | CAR-T Cell Medium (Human Serum) | 500 ML | $999 | |
| PM-CAR2001 | CAR-T Cell Medium (Fetal Bovine Serum) | 500 ML | $659 | |
| PM-CAR2002-2ML | CD28/CD3 PM-T-Cell Activation/Expansion Beads | 2 ML | $599 | |
| PM-CAR2002-10ML | CD28/CD3 PM-T-Cell Activation/Expansion Beads | 10 ML | $1,900 | |
Under Current Development
| Her-3(Erb-3) | Her-4(Erb-4) | CEA | EGFRviii | PSMA | CD20 |
| VEGFR-1 | VEGFR-3 | c-Met | Lewis A | ROR-1 | CD326 |
| CD133 | CD19 CAR+PD-1 siRNA | CD19+ TIGIT siRNA | BCMA-CS1 | BCMA-CD33 | NKG2d |
| MUC1 | PSCA | PSA | CA-125 (MUC16) | Notch | FLT-3 |
CAR Detection Probes
| Catalog # | Description | Unit Size | Price | DataSheet |
| PM-AB001 | Beam1 Antibody | 100ul, 1 vial | $449 | |
| PM-AB002 | Beam2 Antibody | 100ul, 1 vial | $449 | |