CAR-T cells

CAR-T Cells

Promab Biotechnologies has generated CARs (chimeric antigen receptors) specific for a variety of target antigens, many of which are overexpressed on tumor cells. These target antigens include CD19, CD22, CD133, Her-2, EGFR, Mesothelin, and more (see Table, below). In addition, Promab has modified the CARs in a number of ways to increase their utility, including adding inducible caspase-9 as a safety switch; adding non-immunogenic tags to provide for CAR identification and selection; and using different costimulatory domains to modulate CAR functional responses.

Promab Biotechnologies uses high-titer lentivirus transduction to introduce the CARs into T cells, generating CAR-T cells. Promab has also generated CAR-NK cells and CAR-macrophages for a subset of CARs. The CAR-T cells are validated using multiple methods, including flow cytometry (to determine the CAR-T frequency and phenotype), real-time and end-point cytotoxicity assays, cytokine production and checkpoint protein upregulation.

For a negative control, Promab has generated a mock CAR which lacks the target-recognizing scFv domain but can be detected with the Beam-2 antibody. Promab also offers non-transduced T cells as another negative control.

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Promab Biotechnologies has generated the following CAR-T products:

CAR-T Cells

Catalog # Description Cell Number Price DataSheet
PM-CAR1000-1M Mock scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1001-1M CD19 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1002-1M CD19 scFv-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1003-1M CD19 scFv-CD28-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1004-1M CD19 scFv-CD28-4-1BB 1x10 6 /vial $1,000 pdf icon
PM-CAR1005-1M CD19scFv-TM-41BB-CD3z-T2A-GFP 1x10 /vial $1,000 Coming Soon
PM-CAR1006-1M iCas9-T2A-antiCD19 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1007-1M CD19 scFv-FLAG-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1008-1M no scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1009-1M CD19 scFv-CD28 1x10 6 /vial $1,000 pdf icon
PM-CAR1010-1M iCas9 HA-T2A-antiCD19scFv-CD28-CD3ζ−GGGS-FLAG 1x10 6 /vial $1,000 pdf icon
PM-CAR1011-1M Mesothelin scFv-CD28-CD3ζ  1x10 6 /vial $1,000 pdf icon
PM-CAR1012-1M Mesothelin scFv-CD28-4-1BB-CD3ζ (3rd) 1x10 6 /vial $1,000 pdf icon
PM-CAR1013-1M Mesothelin scFv-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1014-1M Mesothelin scFv-FLAG-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1015-1M Mesothelin scFv-4-1BB-CD3ζ-FLAG 1x10 6 /vial $1,000 pdf icon
PM-CAR1016-1M VEGFR2 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1017-1M GPC3 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1018-1M CD133 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1019-1M EpCAM1 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1020-1M EpCAM scFv-CD28-CD3ζ * NheI restriction site introduced, N-terminal of scFV (amino acids: AS) 1x10 6 /vial $1,000 pdf icon
PM-CAR1021-1M EGFR scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1022-1M EGFR scFv-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1023-1M EGFR scFv-TM28-GITR-CD3ζ 1x10 /vial $1,000 pdf icon
PM-CAR1024-1M Her2 scFv-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1025-1M EGFR scFv-TM28-CD3ζ-GITR 1x10 6 /vial $1,000 pdf icon
PM-CAR1026-1M CD33 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1027-1M CD38 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1028-1M CD138 scFv-Beam1-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1029-1M CD22 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1030-1M CD22 scFv-TM8-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1031-1M BCMA-4-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1032-1M CD4 scFv-Beam1-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1033-1M Humanized BCMA-2 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1034-1M Mouse ROR-1 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1035-1M CD19 scFv-CD22 scFv-4-1BB-CD3ζ  1x10 6 /vial $1,000 pdf icon
PM-CAR1036-1M Humanized CEA scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1037-1M BCMA-4 scFv-TM8-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1038-1M NGFR scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1039-1M MCAM scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1040-1M CD47 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1041-1M Humanized CD47 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1042-1M Humanized CD19 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1043-1M Humanized CD19 scFv-Beam1-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1044-1M Human ROR1 scFv-TM28-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1045-1M PDL-1 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1046-1M CD123 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1047-1M CD22 scFv-Beam2-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1048-1M CD19 scFv-Beam2-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1049-1M CD19 scFv-CD22 scFv-4-1BB-CD3-T2A-tEGFR (Suicide switch) 1x10 6 /vial $1,000 pdf icon
PM-CAR1050-1M CD19 scFv-CD22 scFv-4-1BB-CD3-T2A-RQR8 (Ritiximab suicide switch) 1x10 6 /vial $1,000 pdf icon
PM-CAR1051-1M BCMA-2 scFv-TM-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1052-1M CD37 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1053-1M CD37 scFv-TM28-4-1BB-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1054-1M CD19 scFv-TM28-GITR-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1055-1M CD19 scFv-TM8-GITR-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1056-1M CD33 scFv-Beam 2-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1057-1M CS1 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1058-1M B7H4 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1059-1M CD24 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1060-1M Mesothelin scFv-TM28-CD28-4-1BB-CD3ζ 1x10 /vial $1,000 pdf icon
PM-CAR1061-1M Beam2-TM28-CD28-CD3ζ (Mock)  1x10 /vial $1,000 pdf icon
PM-CAR1062-1M CD20 scFv-TM28-CD28-CD3ζ 1x10 6 /vial $1,000 pdf icon
PM-CAR1063-1M Her2 scFv-4-1BB-CD3ζ-EGFRt 1x10 /vial $1000 pdf icon
PM-CAR1064-1M Her2 scFv-4-1BB-CD3ζ-GFP 1x10 /vial $1,000 pdf icon
PM-CAR1065-1M c-MET scFv-4-1BB-CD3ζ 1x10 /vial $1000 Coming Soon
PM-CAR1066-1M CD19 scFv-FLAG-CD28-CD3ζ-EGFRt 1x10 /vial $1,000 Coming Soon
PM-CAR1067-1M CD19 scFv-FLAG-4-1BB-CD3ζ-EGFRt 1x10 /vial $1000 Coming Soon
PM-CAR1068-1M EpCAM scFv-TM-4-1BB-CD3ζ 1x10 /vial $1,000 Coming Soon
PM-CAR1069-1M PSMA scFv-4-1BB-CD3ζ 1x10 /vial $1000 Coming Soon
PM-CAR1070-1M Her2 scFv-4-1BB-CD3ζ 1x10 /vial $1,000 Coming Soon
PM-CAR1071-1M CD19 scFv-4-1BB-CD3ζ-GFP 1x10 /vial $1,000 Coming Soon

CAR Macrophage Cells

Catalog # Description Cell Number Price DataSheet
PM-CARMA01 CD19 scFv-Flag CAR THP-1 Cells 1x10/vial $6,000 pdf icon
PM-CARMA02 Mesothelin scFv CAR THP-1 Cells 1x106/vial $6,000 pdf icon

Non-Transduced T Cells

The non-transduced T cells are available in frozen vials that can be activated and expanded with CD28/CD3 beads and used as a negative control in all assays with CAR-T cells. Promab Biotechnologies has non transduced T cells from different donor that can be used in the assay to test individual differences among different donors.

Catalog # Description Cell Number Price DataSheet
PM-CAR2003 Non-transduced T cells, healthy donor 1x106 /vial $500 pdf icon
PM-CAR2004 Non-transduced T cells, ALL 1x106/vial $500 pdf icon
PM-CAR2005 Non-transduced T cells, CLL 1x106/vial $500 pdf icon
PM-CAR2006 Non-transduced T cells, NHL 1x106/vial $500 pdf icon
PM-CAR2007 Non-transduced T cells CD4T cells 1x106/vial $1,000 pdf icon
PM-CAR2008 Non-transduced T cells CD8T cells 1x106/vial $1,000 pdf icon
PM-CAR2009 Non-transduced T CD4Tcm cells  1x106/vial $1,000 pdf icon
PM-CAR2010 Non-transduced CD4Tem cells   1x106/vial $1,000 pdf icon
PM-CAR2011 Non-transduced Tnaïve cells  1x106/vial $1,000 pdf icon
PM-CAR2012 Non-transduced Tcm cells 1x106/vial $1,000 pdf icon
PM-CAR2014 Non-transduced T cells, GFP+ 1x106/vial $1,000 pdf icon

Non-Transduced Macrophages

Catalog # Description Cell Number Price DataSheet
PM-CAR2013 Non-transduced Macrophage Cells 1x106/vial $500 pdf icon

Engineered CAR-T Target Cells

Promab Biotechnologies has developed several target cell lines to be used in cytotoxicity assays with CAR-T effector cells. These cells express tumor antigen that is overexpressed in malignant cells, can have GFP or luciferase for in vivo studies, or knockout cell lines by CRISPR.

Catalog # Description Marker Cell Number Price Datasheet
PM-Raji-GFP Raji-GFP stable expression GFP+CD19+ 1x106/vial $6,000 pdf icon
PM-Jurkat-GFP Jurkat-GFP stable expression GFP+, CD19 1x106/vial $6,000 pdf icon
PM-K562-GFP K562-GFP stable expression GFP+, CD19- 1x106/vial $6,000 pdf icon
PM-CD19-HELA CD19-HeLa stable expression CD19+ 1x106/vial $6,000 pdf icon
PM-CD19-CHO CD19-CHO stable expression CD19+ 1x106/vial $8,000 pdf icon
PM-CD47-CHO CD47-CHO stable expression CD47+ 1x106/vial $8,000 pdf icon
PM-BxPC3-GFP-LUC BxPC3-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-BCMA-CHO BCMA-CHO stable expression BCMA+ 1x106/vial $8,000 pdf icon
PM-CD22-CHO CD22-CHO stable expression CD22+ 1x106/vial $8,000 pdf icon
PM-CS1-CHO CS1-CHO stable expression CS1+ 1x106/vial $8,000 pdf icon
PM-RPMI8226-GFP-LUC RPMI8226-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-H929-GFP-LUC H929-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-SKOV-3-GFP-LUC SKOV3-GFP-LUC stable expression GFP+, LUC+ 1x106/vial $6,000 pdf icon
PM-CD37-CHO CD37-CHO stable expression CD37+ 1x106/vial $8,000 pdf icon
PM-CS1-HELA-GFP CS1-HeLa-GFP stable expression GFP+ 1x106/vial $6,000 pdf icon
PM-BCMA-K562-GFP BCMA-K562-GFP stable expression
GFP+ 1x106/vial $6,000 pdf icon
PM-BCMA-HELA-GFP BCMA-HeLa-GFP stable expression GFP+ 1x106/vial $6,000 pdf icon
PM-SKOV3-ROR1-KO ROR1 knockout SKOV3 cell line ROR1- 1x106/vial $6,000 pdf icon
PM-HeLa-ROR1-KO ROR1 knockout HeLa cell line ROR1- 1x106/vial $6,000 pdf icon

PBMC

Catalog # Description Cell Number Price DataSheet
PM-CAR3000 PBMC, ALL 1x106/vial $500 pdf icon
PM-CAR3001 PBMC, CLL 1x106/vial $500 pdf icon
PM-CAR3002 PBMC, NHL 1x106/vial $500 pdf icon

Cell Media and Activation Beads

Catalog # Description Volume/Unit Price DataSheet
PM-CAR2000 CAR-T Cell Medium (Human Serum) 500 ML $899 pdf icon
PM-CAR2001 CAR-T Cell Medium (Fetal Bovine Serum) 500 ML $599 pdf icon
PM-CAR2002-2ML CD28/CD3 PM-T-Cell Activation/Expansion Beads 2 ML $599 pdf icon
PM-CAR2002-10ML CD28/CD3 PM-T-Cell Activation/Expansion Beads 10 ML $1,600 pdf icon

Under Current Development

Her-3(Erb-3) Her-4(Erb-4) CEA EGFRviii PSMA CD20
VEGFR-1 VEGFR-3 c-Met Lewis A ROR-1 CD326
CD133 CD19 CAR+PD-1 siRNA CD19+ TIGIT siRNA BCMA-CS1 BCMA-CD33 NKG2d
MUC1 PSCA PSA CA-125 (MUC16)

Notch

FLT-3

CAR Detection Probes

Catalog # Description Unit Size Price DataSheet
PM-AB001 Beam1 Antibody 100ul, 1 vial $399 pdf icon
PM-AB002 Beam2 Antibody 100ul, 1 vial $399 pdf icon
PM-AB003 BCMA Biotinylated Protein 50ug, 1 vial $499 pdf icon
PM-AB004 Mesothelin Biotinylated Protein 50ug, 1 vial $499 pdf icon