| phase | Week | Description of Process | Included | Additional Cost | |
|---|---|---|---|---|---|
| Phase 0 (Initial Design) | Week 1 | Peptide Design | ✔ | ||
| Peptide Synthesis | $750 | Request a Quote | |||
| Peptide Conjugation | $300 | Request a Quote | |||
| Synthesis + Conjugation | $1000 | Request a Quote | |||
| Phase I (Immunization) | Week 2 | Immunization of 5 mice | ✔ | ||
| Immunization of 10 mice or more | $300 setup fee $50/mouse | Request a Quote | |||
| Week 4 | 2nd Injection of antigen (immunization boost) | ✔ | |||
| Week 6 | 3rd Injection of antigen (immunization boost) | ✔ | |||
| Week 7 | Polyclonal sera collected | ✔ | |||
| Phase | Week 7.5 | ELISA evaluation of titer | ✔ | ||
| Phase I | Week 8 Week 9 | 4th Injection of antigen (immunization boost) | ✔ | ||
| Polyclonal sera collected | ✔ | ||||
| Week 4 | ELISA evaluation of titer | ✔ | |||
| Week 10 | 5th Injection of antigen (immunization boost) | ✔ | |||
| Week 11 | Polyclonal sera collected | ✔ | |||
| ELISA evaluation of titer | ✔ | ||||
| Phase II (Cloning) | Week 12 | Picking 10 clones from a pool of 3000 clones | ✔ | $3,595 | |
| Picking 11 clones or more | $50/clone | Request a Quote | |||
| Negative screening – testing to make sure clones don’t cross-react | $500/antigen | Request a Quote | |||
| Screening with Western Blot | ✔ | $500 | |||
| Screening with different applications (IF, FC, IHC) |
✔ | $500/application | Request a Quote | ||
| Phase III (Subcloning) | Week 13 | 2 sub-clones will be selected, isotyped and expanded into T25 flasks | ✔ | $1,400 | |
| Picking 3 sub-clones or more | ✔ | $500/sub-clone | Request a Quote | ||
| Cell line adaptation to serum free media | $1200/sub-clone | Request a Quote | |||
| Week 14 | Cryopreservation of hybridoma cells and shipment within the U.S.A. | ✔ |
Mouse Monoclonal Antibodies
ProMab Biotechnologies dedicated itself from the first day to develop monoclonal antibodies almost exclusively.We have an onsite vivarium and working to establish a GMP facility soon. We keep a veterinarian on staff to make sure our mice and other animals are in the best health.
Included in Custom Package
- Peptide Design
- Immunization of 5 Balb/c mice
- Western Blot screening with antigen
- Evaluation of titer by ELISA
- Screening of 10 clones by ELISA from an initial pool of 1000 clones
- Sub-cloning and expansion of 2 clones
- Antibody isotyping
- 2 vials of hybridoma cells cryopreserved and stored for 6 months
We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application.
Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide your through the 10 to 14-week process.
PHASE I
* It is possible for certain antigens and certain mice to have stronger immune response than others. It typically takes 5 injections of antigen into mice for a mature immune response. Sometimes additional immunization boosts are required which can add another 2 to 4 weeks to the project. There are also times when it only requires 3 immunization boosts to get the desired titer.
Supernatant from 10 clones (or more if selected) will be sent to the client for in-house evaluation and use feedback to select 2 sub-clones (or more if selected). The Custom Mouse Monoclonal Antibody Package starts at $6,995.
PHASE III
Additional Add-On Services
| Ascites Production ($300 hybridoma setup fee) |
< 11 mice | $60/mouse | Request a Quote |
| 11 - 50 mice | $55/mouse | Request a Quote | |
| 51 – 99 mice | $50/mouse | Request a Quote | |
| >100 mice | $45/mouse | Request a Quote | |
| Ascites Purification | Any number of mice | $60/mouse | |
| Hybridoma Cells Expansion (Antibody Production) | Hybridoma cells in bioreactor (1 liter of supernatant) | $3,200/clone | Request a Quote |
| Hybridoma cells in bioreactor (500ml of supernatant) | $2,600/clone | Request a Quote | |
| Antibody Purification (supernatant into 1 mg/ml solution) | 1 liter | $1000 | Request a Quote |
| 100 ml | $400 | Request a Quote | |
| 1 ml | $40 | Request a Quote | |
| Antibody Labeling | FITC | $1000 | Request a Quote |
| HRP | $1000 | Request a Quote | |
| Biotin | $1000 | Request a Quote | |
| Epitope Mapping | Identify the exact epitope of the antigen – antibody binding site | Please inqiure | Request a Quote |
| Cold Storage (Liquid Nitrogen) | 2 vials after 6 months | $60/month | Request a Quote |
| 3 vials or more | $30/vial/month | Request a Quote |
Other Custom Services:
Rabbit Monoclonal Antibodies
Human monoclonal antibodies
Hybridoma Sequencing