ProMab Biotechnologies Custom T Cell Activation and Proliferation
T cell activation is initiated by the engagement of TCR and CD3 complex, and the subsequent engagement of co-stimulatory molecules, such as CD28 receptor. After co-stimulation of these two signals, a series of intracellular activations happen, including the cytokine release and T cell proliferation in order to fight infection or disease.
ProMab has developed a systematic approach to T cell activation and proliferation assays for IO products discovery. The nuclear factor of activated T cells (NFAT), IL-2 or other kinds of factors will be detected by bioluminescent methods or ELISA kits. Meanwhile, the T cell activation and proliferation process can also be evaluated via cell counting kit-8 (CCK-8), MTT, CFSE as well as flow cytometric analysis, etc.