P16 (Mouse and Human) Primary Antibody

Item Information
Catalog # Size/Concentration Price
20129 100μl $412.00
Product OrderP
Entrez GeneID1029
FormulationAscitic fluid containing 0.03% sodium azide.
ImmunogenPurified recombinant fragment of P16 expressed in E. Coli.
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman, Rat
IHC_P (Immunohistochemistry) 1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000

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Western Blot

Cell Culture Products
Figure 1: Western blot analysis using P16 mouse mAb against truncated P16 recombinant protein.

Immunohistochemical analysis

Cell Culture Products
Figure 2: Immunohistochemical analysis of paraffin-embedded rat liver tissue (A), human brain tissue (B) and brain tumor (C), showing nuclear localization using P16 mouse mAb with DAB staining.
Product Overview
Description The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.
References (references)
References (references) 1. Hunter, T. 1993. Cell 75: 839-841.
2. Sherr, C.J. 1993. Cell 73: 1059-1065.
3. El-Deiry, W.S., et al. 1993. Cell 75: 817-825.

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