EGFP mRNA-LNP

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Description

This product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of EGFP mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The LNP’s used are formulated with, SM-102, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. EGFP is an enhanced version of GFP (green fluorescent protein), originally isolated from Aequorea Victoria, and shows a bright green fluorescence with an emission peak of 509nm. EGFP is a useful marker for tracking gene expression and transfection efficiency in mammalian cultures. The Sequence of EGFP used is available upon request.

Product Overview

Composition

mRNA isolated in sodium citrate, sodium acetate, lipid mix in ethanol, and PBS.

Cell Type Tested

HEK293S, iPSC, T cells

Application & Handing

Store at 4°C. Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice. Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.

Storage

4°C; ice

Product Image

GFP Expression in T Cells

Figure 2: GFP expression of T cells transfected by PM-LNP-0021, checked by FACS at 24 hours post transfection. Gated by non-transfected T cells under identical culture conditions.

For Research Use Only. Not for use in diagnostic procedures.