Flow Cytometry Services

By breaking down tissue samples, and purifying them, we can look at individual cells, e.g white blood cells or liver cells. By further lysing the cells, we can look at the individual cellular components, e.g. nuclei, mitochondria. With flow cytometry we can detect the presence of a certain kind of cells we are looking for. With flow cytometry we can measure the concentration of a given component in relation to the total volume of the sample. Flow cytometry gives information on protein localization (based on isolated region), cell viability (based on proportion of intact and degraded DNA), metabolic capacity (based on nu of mitochondria). See various applications and examples. >>> Flow Cytometry Applications

On-site, ProMab offers flow cytometry on the following instruments:

BD FACS Calibur with blue and red lases, 4 color fluorescent parameters

Acea Novocyte with blue, red and violet lasers with 13 color fluorescent parameters and high-throughput sampler.

Through our partners we have the added capability of tube and plate-based cell sorting and additional fluorescent parameters.

Flow Cytometry Data Acquisition 

We can process samples for you on-site before data acquisition. We can perform flow cytometry acquisition on samples processed by our customers already or sent to us from a third-party lab.

Flow Cytometry Data Analysis

We use FlowJo 10 for data analysis. We offer complex gating strategies customized to your needs.