Stable Cell Line Development
ProMab Biotechnologies has 2 decades of experience
generating highly specific and potent monoclonal antibodies. We have generated
thousands of hybridoma cell lines for our catalog and custom projects for
clients from academic institutions and pharmaceutical companies. We have
expanded our offerings to provide a proprietary CHO-K1 stable cell line
development service for research and biopharma applications.
Stably-transfected cell lines are used for many drug discovery assays such as
screening and validation. Compared to traditional antibody production methods,
utilizing a stable cell line overcomes many production issues and
inconsistencies that come with hybridoma production or standard transient
expression. For cost-effective large scale production, a stable cell line is
the way to express your gene of interest. A stable cell line allows for
persistent and reproducible expression for your gene of interest over many
passages.
ProMab offers a complete package for stable cell line development utilizing
our proprietary CHO-K1 expression vector or we can start from yours. The time
consuming and strenuous process will be handled by our team of experts to
develop the stable cell lines you need for protein production and cell-based
assays. Further assay development and characterization can also be done at our
R&D facility, including but not limited to flow cytometry,
immunohistochemistry, western blot analysis, SPR, and many more.
Included in custom stable cell line engineering service:
- Transfection
- Selection from pool of transfected cells
- Clonal screening and selection
- Cell line characterization
- Expansion and downstream evaluation
Antibody & Protein Stable Cell Line Development
Stable Cell Line Production | Cost |
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DNA reconstruction of recombinant antibody in patented plasmid
vector. Transfection into high expressing system of a human cell line. Single cell screening and clone selection for the highest producing clone. Production: Antibody expression and purification from 500 mL culture. |
Inquire |
Master and Working Cell Banking Services | Inquire |