ProMab Biotechnologies dedicated itself from the first day to develop
monoclonal antibodies almost exclusively. We have an onsite vivarium and
working to establish a GMP facility soon. We keep a veterinarian on staff to
make sure our rabbits and
other animals are in the best health.
Starting at $2,450
We know how to generate an antibody that is specific to the immunizing
antigen and that works for the intended application.
Our technical staff with decades of experience in immunology,
biochemistry, and molecular biology will be there to guide your through
the 9 to 12-week process.
Immunization and anti-sera production:
*Conjugated peptides or recombinant proteins are used to immunize 2
rabbits. (See Add-on Services)
- Injection done every 2 weeks (4 to 6 injections)
Pre-immune (5 ml) and immune bleeds (100-120 ml antiserum) are collected
ELISA (and Western blot for protein antigen) to determine the titer
time: 9-12 weeks Price: $1,950
Antibody will be precipitated from sera using ammonia persulfate and
suspended in PBS containing 0.03% sodium azide.
- Estimated time: 3 days
- Price: $500
* It is possible for certain antigens and certain rabbits to
have stronger immune response than others. It typically
takes 5 injections of antigen into rabbits for a mature immune
response. Sometimes additional immunization boosts are
required which can add another 2 to 4 weeks to the project.
There are also times when it only requires 3 immunization
boosts to get the desired titer.
*Antigen Preparation-Recombinant protein production
Peptide synthesis & conjugation
Keep rabbits alive beyond good titer (cost per rabbit per
Peptide or protein affinity purification
Other Custom Services: