Mammalian Expression

ProMab Biotechnologies, Inc. offers a recombinant protein expression service in mammalian cells. Our high-level mammalian expression service allows you to scale up to 50 liters, request tag removal and is from a human cell line origin with full glycosylation.
 
Below is the breakdown for the standard e. coli expression service by phase. We also offer protein expression services including E. coli Expression and Baculovirus Expression. For additional inquires on any of our protein expression services please contact us and we would be glad to answer any of your questions.
 
 
  • High-level expression system.
  • Competitive Price.
  • Human cell line origin with full glycosylation.
  • Fast turn-around time.
  • Scale up to 50 liters.
  • Tag removal as requested.

Breakdown of Standard Service by Phase

Phase I

  • cloning of your gene of interest into vector with his-tag or IgG-Fc tag (human or murine)
  • Sequence confirmation of insert
  • Small scale (10 mL) transfection to test the expression level; and subsequent optimization if necessary

$2,000 due at the beginning of phase
Estimated time: 3-4 weeks

Phase II

  • Mid-scale transfection (50mL) and purification
  • SDS-page validation

$2,500 due at the beginning of phase.
Estimated time: 2-3 weeks

Phase III

  • Large scale transfection (1L) of recombinant vector into HEK293 or CHO cells, and protein expression, evaluation and optimization
  • Protein purification from 500 mL of HEK293 or CHO cell culture using Ni-NTA or Protein G affinity purification columns (tag cleavage provided at additional cost).

$3,500 due at the beginning of phase
Estimated time: 4 weeks

Yield ranges from 200 µg-25 mg/L of culture with average yield of 3mg/L
Total time = 9-10 weeks ±
Total cost= $8,000

Deliverables: 5 μg of transfer vector, protein, and documentation.