A simple “copy-and-paste” approach to introduce a rodent CDR into a human antibody scaffold will often diminish or destroy the binding specificity and affinity of the original rodent antibody. Humanization therefore will rely on discrete CDR residue substitutions to obtain an optimum balance of minimal immunogenicity toward the antibody, while maintaining the original binding and recognition characteristics.
ProMab can humanize your already established mouse monoclonal antibody or start from scratch if you are interested in antibody humanization.
