Menu
  • About Us
  • News
  • Publications
  • Events
  • Careers
  • Contact
ProMabLogoProMabLogoProMabLogoProMabLogo
  • Login
  • 0 Items ($0.00)
    My Cart 1
  • Login
  • 0 Items ($0.00)
    My Cart 1
  • Top Sellers
    • CD19scFv-BEAM-CD28-CD3ZCD19scFv-BEAM-CD28-CD3ζ
    • CAR-T_Cell_MediumCAR-T Cell Medium
    • CD28-CD3_PM-T-Cell_Activation__Expansion_Beads_editCD28-CD3 Activation Beads
    • Cancer._Stem_PremiumCancer Stem Premium
    • P16_Antibody-v2P16 Antibody
  • Products
    • COVID19_8-04-2020COVID-19
    • primary-antibodyPrimary Antibody
      • Polyclonal Antibodies
      • Monoclonal Antibodies
    • Protein_IconFull-Length Protein
      • Recombinant Proteins
      • Natural Proteins
      • COVID-19 Research Products
    • CAR-T_iconCAR-T/CAR- NK
      • CAR-T cells
      • CAR-NK cells
      • T cell Activation Beads
      • Peripheral Blood Monocytes
    • Hempotological_products_IconHematological Products
      • Primary Cells
      • Non-Transduced T cells
      • Cell Media
      • Freezing Media
    • Icon_Product_mRNP_Lipid Nanoparticle_5_02_2022_finalmRNA-Lipid Nanoparticles
      • mRNA Products
      • LNP Products
    • stem-cell-iconCancer Stem Cells
      • Cancer Stem Cell Media
      • Hepatocellular Carcinoma (HCC)
      • Kidney Cancer
      • Ovarian Adenocarcinoma
      • Pancreatic Cancer
      • Prostate Cancer
      • Renal Cell Carcinoma
      • Lung Cancer Non-Small Cell (NSCLC)
      • Bladder Cancer
      • Breast Carcinoma
      • Colorectal Cancer
      • Glioblastoma
      • Head and Neck (H&N) Carcinoma
  • Services
    • v1-_Licensing_and_Distributing_CAR_Technology_7_22_2019_edit-1Licensing and Distributing CAR Technology
    • No_circles_-v2_Engineered_Cell_Lines-7_22_2019_finalCAR-T/CAR-NK Development
      • Custom CAR-T Cell Development
      • Custom CAR-NK Cell Development
      • Custom CAR-Macrophage Cell Development
      • Custom Gamma Delta T Cell Development
      • mRNA-LNP Gene Delivery
      • Lentivirus Production
      • Animal Research
      • Cancer Stem Cell Services
    • Cell_Based_Assay1_lastCell Based Assays
      • Flow Cytometry Services –
      • T Cell Specificity Assay
      • T Cell Activation & Proliferation Assay
      • Responsive T Cell Trafficking Assay
      • Cytokine Response Assay
      • Macrophage Polarization Assay
      • DC Migration Assay
    • Icon_ Services_mRNP_Lipid Nanoparticle_5_02_2022_finalmRNA-Lipid Nanoparticles
      • mRNA-LNP Gene Delivery
    • No_circles_-v3-Custom_Antibody_Engineering_7_22_2019_finalAntibody Engineering Services
      • Mouse Monoclonal Antibodies
      • Rabbit Monoclonal Antibodies
      • Human Monoclonal Antibodies
      • Recombinant Antibodies
      • Bispecific Antibodies
      • Antibody Humanization
      • Rabbit Polyclonal Antibodies
      • Hybridoma Sequencing
      • Antibody Production
    • No_circles_-v4-Protein_Expression_Services_7_22_2019_finalProtein Expression Services
      • E.coli Expression
      • Baculovirus Expression
      • Mammalian Expression
      • Stable Cell Line Development
      • Peptide Synthesis
      • Gene Synthesis Services
  • Protocols & Troubleshooting
    • Protocols
    • Technology
    • Troubleshooting
    • FAQ

Macrophage Polarization Assay

Account
  • Home
  • Macrophage Polarization Assay
    • Cell Based Assays
    • Flow Cytometry Services
    • Cytokine Release Assays
    • T Cell Specificity Assay
    • T Cell Activation & Proliferation Assay
    • Responsive T Cell Trafficking Assay
    • Macrophage Polarization Assay
    • DC Migration Assay
    • mRNA-Lipid Nanoparticle Gene Delivery

ProMab Biotechnologies offers custom assays to induce different macrophage subsets and characterize their polarization states.

Request A Quote

ProMab Biotechnologies Custom Macrophage Polarization Assays

Macrophages exert multiple functions including modulating the adaptive immune responses and responding to pathogens. Macrophages show specific functions and phenotypes in response to different stimuli. They can display polarized states via their plasticity and different populations of macrophages can be developed in response to different stimuli.

The macrophage polarization has been classified as being either M1 (pro-inflammatory) or M2 (anti-inflammatory) state, which is further divided into M2a, M2b, M2c and M2d subsets.

Figure 1. The main polarization states of activated macrophages. (Atri, 2018)

Our assay platform can generate various macrophage subsets by treating isolated bone marrow-derived or peritoneal macrophages with specific stimuli, such as IFN-γ (M1), IL-4 (M2a), LPS and IL-1β (M2b) or IL-10 (M2c). In addition, we also provide the verification of each polarized macrophage based on the distinctly produced cytokines or expressed surface markers. The analysis of cytokines expression and surface markers is usually carried out by ELISA or flow cytometry, upon request.

Each macrophage polarization state demonstrates a unique cytokine profile and cell surface marker profile that can be used to characterize the macrophage populations in tissue inflammatory conditions to further understand disease pathogenesis and support the cell and gene therapy-based IO drugs development. Specifically, different macrophage populations can be generated by different stimuli, for example, IFNγ, LPS, IL-4, IL-13, IL-10, IL-6, glucocorticoids, adenosines and immune complexes, etc.

Contact us today for a proposal to support all of your Macrophage Polarization needs.

Request A Quote

References

  1. Iqbal, S. and Kumar, A. (2015). Characterization of In vitroGenerated Human Polarized Macrophages. J Clin Cell Immunol. 6:6.
  2. Atri, C.;et al. (2018). Role of Human Macrophage Polarization in Inflammation during Infectious Diseases. International Journal of Molecular Sciences. 19(6), p.1801.

Other Components of ProMab's Cell Based Assay Platform: 

  • Flow Cytometry
  • T cell Specificity Assay
  • T cell Activation & Proliferation Assay
  • Responsive T Cell Trafficking Assay
  • DC Migration Assay
  • Macrophage Polarization Assay
  • Cell Line Engineering

Other Custom Services:

mouse
Mouse monoclonal antibodies
human
Human monoclonal antibodies
hybridoma_sequencing
Hybridoma Sequencing

Sign up for news & promotions:

Products

  • Antibodies
  • Proteins
  • CAR T/NK Cells
  • Cell Lines
  • Cell Culture

Services

  • CAR-T/CAR-NK Development
  • Custom Antibodies
  • Synthesis and Expression
  • Clinical Research

Resources

  • Protocols
  • Technology
  • Troubleshooting
  • FAQ

Promab Biotechnologies

  • Promotions
  • About
  • News
  • Publications
  • Careers
  • Contact Us

Follow Us

Copyright © ProMab 2022 All Rights Reserved ProMab Biotechnologies, Inc. | 2600 Hilltop Drive, Building B, Suite C320, Richmond, CA 94806 USA | Email: info@promab.com