Starting at $21,000
We know how to generate an antibody that is specific to the immunizing antigen and that works for the intended application.
Our technical staff with decades of experience in immunology, biochemistry, and molecular biology will be there to guide your through the 24 to 28- week process.
|Phase I *||Immunization of rabbits with recombinant protein or KLH conjugated peptide.
Isolation of rabbit peripheral blood mononuclear- cells (PBMC) from the animal with the highest ELISA-positive titer, with subsequent screening and sorting of antigen-positive, and IgG-positive B cells
|Phase II||Total RNA isolation from sorted, antigen-positive B cells, and preparation of surface-display library.||$5000|
|Phase III||Multi-round screening of display-library by flow cytometry and panning.||$6000|
|Phase IV||Generation of candidate full-length Heavy-chain and Light-chain clones, expression of full-length and complete recombinant rabbit monoclonal antibodies, and validation to the required applications||$6000|
* It is possible for certain antigens and certain rabbits to have stronger immune response than others. It typically takes 5 injections of antigen into rabbits for a mature immune response. Sometimes additional immunization boosts are required which can add another 2 to 4 weeks to the project. There are also times when it only requires 3 immunization boosts to get the desired titer.