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TIM-3 (T-cell immunoglobulin and mucin domain-containing protein 3), also known as HAVCR2 (hepatitis A virus cellular receptor 2), encoded by the HAVCR2 (TIM-3) gene, is a member of the TIM family of immunoregulatory proteins. The proteins of this family share a common structural organization consisting of an amino-terminal immunoglobulin variable domain with five atypical cysteines, a mucin stalk, a transmembrane domain, and a cytoplasmic tail. As a transmembrane receptor, TIM-3 has been found to have a variety of different ligands that bind to different regions on the extracellular immunoglobulin V domain of TIM3. By binding to its ligands such as Gal-9 and PtdSer, TIM-3 has been shown to modulate immune responses in autoimmunity and cancer. It has been reported that TIM-3 is mainly expressed on activated CD8+ T cells and inhibits macrophage activation after inhibition of PD-1. TIM-3 expression is upregulated in tumor infiltrating lymphocytes of different types of cancer. As an immune checkpoint, TIM-3 cooperates with the PD-1 receptor to down-regulate the activity of anti-tumor T cells, and the combination of ICI targeting TIM-3 and PD-1 has a significantly better effect on tumor prevention.
This product is designed as a tool for the delivery and expression of TIM-3-TF mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of TIM-3-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The TIM-3-TF fusion protein is approximately 32 kD, consisting of mature TIM-3 (280 amino acids) and a C-terminal TF tag (15 amino acids). The mature peptide sequence of TIM-3 is 22-301 of 301 amino acids, GenPept accession number is NP_116171.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.