Item Information
Catalog #

SIGLEC7, also known as CD328, is a protein belonging to the sialic acid-binding immunoglobulin-like lectin (SIGLEC) family, encoded by the SIGLEC7 gene. Proteins of this family are expressed on the surface of immune cells, are a subset of type I lectins, and bind sialic acid. These SIGLEC proteins have been designated as glycoimmune checkpoints. Their ligands are sialylated glycan proteins that are abnormally expressed on tumor cells. Inhibitory SIGLECs (e.g. SIGLEC-7 and -9) are found on immune cells such as natural killer (NK) and T cells by interacting with their ligands and inhibit immune signaling pathways to dampen immune responses. Studies have found that SIGLEC-7 is associated with a reduction in CD8+ T cell effector functions, including cytotoxicity and cytokine production. SIGLEC-7 was found to be highly expressed in primary leukemia cells and stem cells from patients with acute myeloid leukemia (AML). This may adversely affect the effector responses of SIGLEC-7+ CD8+ T cells in the blood and bone marrow of AML patients.
This product is designed as a tool for the delivery and expression of human TF tagged SIGLEC7 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of SIGLEC7-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The SIGLEC7-TF fusion protein is approximately 53 kD, consisting of SIGLEC7 (467 amino acids) and a C-terminal TF tag (15 amino acids). The GenPept accession number for SIGLEC7 is NP_055200.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.