Nanog mRNA-LNP

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Description

The NANOG gene is important for the acquisition of pluripotency in embryonic stem cells and embryonic germ cells (EGCs). NANOG is a transcription factor that acts as a key factor in maintaining pluripotency and function, and cooperates with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. Since it was found to be highly expressed in cancer stem cells, NANOG may function as an oncogene to promote carcinogenesis. High expression of NANOG is thought to correlate with poor survival of cancer patients. This product is designed as a tool for the delivery and expression of NANOG mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of NANOG mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The NANOG protein is about 34 kD and consists of 305 amino acids. The GenPept accession number is BAC76999.

Product Overview

Composition

mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).

Cell Type Tested

HEK293S

Application & Handing

Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage

4°C; ice

For Research Use Only. Not for use in diagnostic procedures.