mCherry/Luciferase mRNA-LNP

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Description

mCherry is a member of the monomeric red fluorescent protein (mRFP) mFruits family, derived from DsRed of the Discosoma sea anemone. mCherry is a fluorescent protein chromophore used as a tool to visualize genes and analyze their function in experiments. mCherry absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Luciferase is an oxidase that produces bioluminescence and is widely used in biotechnology as a reporter gene. Unlike fluorescent proteins, luciferase does not require an external light source, but requires the addition of the substrate luciferin. This product is designed as a tool for the delivery and expression of mCherry-Luciferase mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of mCherry-Luciferase mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The sequence of the mCherry-Luciferase fusion protein consists of mCherry (236 amino acids) and Luc 550 amino acids) with a GS peptide linker (GGGGSGGGGSGGGGS) between them. The GenPept accession number for mCherry is WIO52923 and the GenPept accession number for Luc is AAA89088.

Product Overview

Composition

mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).

Cell Type Tested

HEK293S, T cells, iPSC

Application & Handing

Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage

4°C; ice

Product Image

FACS Analysis for mCherry Red

Figure 1: HEK293s cells transfected with mCherry RNA-LNP show high expression of mCherry protein. FACS is shown on HEK293s transfected cells

Fluorescent Microscope Analysis mCherry Red-Luc

Figure 2: HEK293s cells transfected with mCherry RNA-LNP show >90% red cells. Upper panel HEK293s cells and HEK293s+Cherry RNA-LNP-transfected cells. Lower panel: Cherry RNA-LNP-transfected HEK293s cells at higher magnificationunder higher magnification.

For Research Use Only. Not for use in diagnostic procedures.