mbIL-2-TF tag (tethered) mRNA-LNP

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Interleukin 2 (IL-2) is an interleukin, a cytokine signaling molecule in the immune system, encoded by the IL2 gene. It is a secreted cytokine that belongs to the IL2 cytokine subfamily that includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. IL-2 is a glycoprotein produced by activated CD4+ and CD8+ T lymphocytes and plays an important role in the proliferation of T lymphocytes and B lymphocytes. IL-2 signaling promotes the activation of various lymphocyte subtypes during cell differentiation through binding to its receptor IL-2R, resulting in an immune or homeostatic response. Due to its role in influencing the differentiation of T helper cells that influence the course of the immune response, IL-2 has been implicated in autoimmune diseases, leading to the development of many therapeutic agents for different autoimmune diseases.
This product is designed as a tool for the delivery and expression of membrane-bound IL-2-TF mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of membrane-bound IL-2-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The membrane-bound IL-2-TF fusion protein is approximately 49 kD, consisting of human IL-2 (153 amino acids) and human IL-2Rα (272 amino acids) with a C-terminal TF tag (15 amino acids) in between. The GenPept accession numbers for human IL-2 and IL-2Rα are NP_000577 and NP_000408 respectively.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.