Luciferase mRNA-LNP

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Description

Luciferase is an oxidase that produces bioluminescence and is widely used in biotechnology as a reporter gene. Unlike fluorescent proteins, luciferase does not require an external light source, but requires the addition of the substrate luciferin. This product is designed as a tool for the delivery and expression of Luciferase mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of Lucifrase mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The sequence of the Luciferase protein consists of 550 amino acids, and the GenPept accession number is AAA89088.

Product Overview

Composition

mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).

Cell Type Tested

HEK293S, T cells, iPSC

Application & Handing

Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage

4°C; ice

Product Image

Luciferase Emmission Reading in HEK293S

Figure 1: Luciferase emission measured in HEK293S cells transfected by PM-LNP-0024, checked by Promega: Steady G-10 Luciferase Assay System. Cells were lysed and luminescence monitored on luminometer instrument.

For Research Use Only. Not for use in diagnostic procedures.