L-myc mRNA-LNP

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Myc is a family of proto-oncogenes that are essential transcription factors, belonging to the superfamily of basic helix-loop-helix (bHLH) DNA-binding proteins. C-myc (MYC), L-myc (MYCL) and N-myc (MYCN) are the three main members of the Myc family involved in cellular metabolism, cell division, differentiation and cell death. After forming a heterodimer with Myc-associated protein X (Max), Myc activates transcription by binding to E-box regions in the target gene promoters. c-Myc plays a fundamental role as a "double-edged sword" that promotes iPS cell generation and malignant transformation, while L-Myc promotes nuclear reprogramming by significantly downregulating the expression of differentiation-related genes. N-Myc-driven neuroendocrine tumors tend to highly express NEUROD1, resulting in enhanced metastatic potential. This product is designed as a tool for the delivery and expression of L-myc mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of L-myc mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The L-Myc protein is about 40 kD and consists of 364 amino acids. The GenPept accession number is NP_001028253.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
Product Image
Expression of L-myc in 293 cells after transfection
Figure 2. FACS with L-myc antibody shows expression of L-myc in HEK293 cells
Figure 2. FACS with L-myc antibody shows expression of L-myc in HEK293 cells
For Research Use Only. Not for use in diagnostic procedures.