IL-7-TF tag mRNA-LNP

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Interleukin 7 (IL-7) is a hematopoietic growth factor encoded by the human IL7 gene. It plays a vital role in health maintenance and disease prevention, and congenital defects in IL-7 signaling lead to severe immune deficiency. IL-7, a molecule known for its growth-promoting effects on B-cell progenitors, remains one of the most widely studied cytokines. IL-7 stimulates the differentiation of pluripotent hematopoietic stem cells into lymphoid progenitors and the proliferation of all cells of the lymphoid lineage (B cells, T cells and NK cells). Clinical trials of recombinant IL-7 as an immunotherapeutic agent for various malignancies have demonstrated its safety and effective immune reconstitution.
This product is designed as a tool for the delivery and expression of TF-tagged human IL-7 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hIL7-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The IL7-TF protein is about 21 kD, consisting of hIL7 and a C-terminal TF tag (15 amino acids). The GenPept accession number for hIL7 (177 amino acids) is NP_000871.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.