IL-2RÎ²-tethered IL-2 mRNA-LNP

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Description

Interleukin 2 (IL-2) is an interleukin, a cytokine signaling molecule in the immune system, encoded by the IL-2 gene. It is a secreted cytokine that belongs to the IL-2 cytokine subfamily that includes IL-4, IL-7, IL-9, IL-15, IL-21, erythropoietin, and thrombopoietin. IL-2 is a glycoprotein produced by activated CD4+ and CD8+ T lymphocytes and plays an important role in the proliferation of T lymphocytes and B lymphocytes through interactions with a multisubunit receptor complex comprising IL2Rα, IL2Rβ, and IL2Rγ. IL-2 has been implicated in autoimmune diseases, leading to the development of many therapeutic drugs for different autoimmune diseases. Systemic IL-2 supplementation enhances immunity against cancer and viral infections. However, clinical trials have shown that high-dose IL-2 infusion can lead to severe side effects like sepsis, while low-dose IL-2 has a short half-life of efficacy. Based on the fact that adult natural killer (NK) cells only express IL2Rβ and IL2Rγ subunits and are therefore relatively insensitive to IL-2, the fusion protein of IL-2 with its receptor IL-2Rβ, expressed by transfection of NK92 cells, can highly activate the transfected cells without additional exogenous IL-2. Tethering IL-2 to its receptor IL-2Rβ opens up new ways to use IL-2 for immunotherapy.
This product is designed as a tool for the delivery and expression of IL-2Rβ-tethered IL-2 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of IL-2Rβ-tethered IL-2 mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The IL-2-2Rβ fusion protein is approximately 77 kD, consisting of human IL-2 (153 amino acids) and human IL-2Rβ (mature peptide: 525 amino acids) with a GS peptide linker (GGGGSGGGGSGGGGS) in between. The GenPept accession numbers for human IL-2 and IL-2Rβ are NP_000577 and NP_000869 respectively.

Product Overview

Composition

mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).

Cell Type Tested

HEK293S

Application & Handing

Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage

4°C; ice

For Research Use Only. Not for use in diagnostic procedures.