IL-23-TF tag mRNA-LNP

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The IL-12beta (IL-12p40) subunit is encoded by the IL-12B gene and the IL-23alpha (IL-23p19) subunit is encoded by the IL-12A gene. As a disulfide-linked complex with the polypeptide p19 binding protein p40, IL-23 is mainly secreted by activated macrophages and dendritic cells located in peripheral tissues. The IL-23 receptor is also a heterodimer of IL-12Rβ1 and IL-23R: the p19 subunit binds IL-23R, while the p40 subunit binds IL-12Rβ1. By binding to the receptor, IL-23 triggers its downstream signaling cascade. Much like IL-12, IL-23 has been implicated in several autoimmune inflammatory diseases, such as colitis, gastritis, psoriasis, and arthritis. However, based on the discovery of p19 and p40 but not IL-12alpha (p35) mRNA, IL-23, rather than IL-12, is the primary cytokine controlling inflammation in peripheral tissues and joints. In addition, IL-23 has been used as one of the therapeutic targets for the treatment of inflammatory diseases. This product is designed as a tool for the delivery and expression of TF-tagged human IL23 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hIL23-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The hIL23-TF protein is about 61 kD, consisting of IL-12beta (p40) and IL-23alpha (p19) with 15aa linker GGGGSGGGGSGGGGS between them and with a C-terminal TF tag (15 amino acids). GenPept accession numbers of human IL-12beta (p40) and IL-23alpha (p19) are NP_002178 and NP_057668.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.