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Interleukin 12 (IL-12) is a p70 heterodimeric cytokine consisting of two subunits, IL-12alpha and IL-12beta, which are encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL-12 is expressed in dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells in response to antigenic stimulation. This cytokine is primarily responsible for the induction and enhancement of cell-mediated immunity and acts to induce TH1 cell differentiation, increase T cell and NK cell activation and cytotoxicity, and suppress or reprogram immunosuppressive cells. In preclinical studies, IL-12 has demonstrated significant antitumor effects against a range of malignancies. But severe toxicities in early clinical trials appear to dampen enthusiasm for IL-12-based immunotherapies.
This product is designed as a tool for the delivery and expression of IL12B-FLAG-VPG-IL12A (secreted) mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of IL12B-FLAG-VPG-IL12A mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The IL12B-FLAG-VPG-IL12A fusion protein is approximately 56 kD, consists of full-length IL-12beta and mature IL-12alpha with a Flag tag (8 amino acids) and VPG peptide (27 amino acids) in between. The GenPept accession numbers for human IL-12beta and IL-12alpha are NP_002178 and NP_000873.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.