IFN-gamma-TF tag mRNA-LNP

Interferon gamma (IFN-γ or IFN-Gamma), encoded by the IFNG gene, is a dimerized soluble cytokine that is the only member of the type II interferon class. IFN-γ is secreted by cells of the innate and adaptive immune systems. It forms homodimers (active form) that bind to the IFN-γ receptor and trigger cellular responses to viral and microbial infections. Mutations in the IFN-γ gene are associated with increased susceptibility to viral, bacterial and parasitic infections, as well as several autoimmune diseases. IFN-γ has been reported to prevent tumor growth, suggesting that this type II interferon could be used in the development of cancer immunotherapies. This product is designed as a tool for the delivery and expression of TF-tagged human IFN-γ mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hIFNγ-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The hIFNγ-TF protein is about 20 kD, consisting of hIFNγ and a C-terminal TF tag (15 amino acids). The GenPept accession number for hIFNγ (166 amino acids) is NP_000610.