eGFP-T2A-mCherry mRNA-LNP

Item Information
Catalog #

Green Fluorescent Protein (GFP) is a protein that fluoresces bright green when illuminated by light in the blue to ultraviolet range. eGFP is a GFP that produces enhanced green fluorescence and is widely used as a reporter gene in cellular and molecular biology. mCherry is a protein of the monomeric red fluorescent protein (mRFP) mFruits family, derived from DsRed of the Discosoma sea anemone. mCherry is a fluorescent protein chromophore used as a tool to visualize genes and analyze their function in experiments. mCherry absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm.
This product is designed as a tool for the delivery and expression of both eGFP and mCherry using eGFP-T2A-mCherry mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of eGFP-T2A-mCherry mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. eGFP-T2A-mCherry mRNA-LNP produces eGFP and mCherry separated by a T2A cleavage peptide. The sequence of eGFP protein consists of 262 amino acids, and the GenPept accession number is CVH74105. The sequence of mCherry protein consists of 236 amino acids, and the GenPept accession number is WIO52923.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.