eGFP mRNA-LNP (v2)
This product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of EGFP mRNA to a variety of mammalian cells, including cell cultures which may typically be prone to difficulties in expressing desired proteins. The LNP’s used are formulated with, Ionizable lipid, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery.
EGFP is an enhanced version of GFP (green fluorescent protein), originally isolated from Aequorea Victoria, and shows a bright green fluorescence with an emission peak of 509nm. EGFP is a useful marker for tracking gene expression and transfection efficiency in mammalian cultures. The Sequence of EGFP used is available upon request.
![Figure 2: GFP expression of T cells transfected by PM-LNP-0021, checked by FACS at 24 hours post transfection. Gated by non-transfected T cells under identical culture conditions.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0021image2.jpg?v=1680113151)