eGFP mRNA-LNP (v1)

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Description

Green Fluorescent Protein (GFP) is a protein that fluoresces bright green when illuminated by light in the blue to ultraviolet range. eGFP is a GFP that produces enhanced green fluorescence and is widely used as a reporter gene in cellular and molecular biology. This product is designed as a tool for the delivery and expression of eGFP mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of eGFP mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The sequence of eGFP protein consists of 262 amino acids, and the GenPept accession number is CVH74105.

Product Overview
Composition
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
HEK293S, T cells, iPSC
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
Storage
4°C; ice
Product Image
GFP Expression in HEK293s Cells
Figure 1: GFP expression of HEK293s cells transfected by PM-LNP-0021, checked by FACS at 24 hours post transfection. Gated by non-transfected HEK293s cells under identical culture conditions.
Figure 1: GFP expression of HEK293s cells transfected by PM-LNP-0021, checked by FACS at 24 hours post transfection. Gated by non-transfected HEK293s cells under identical culture conditions.
GFP Expression in T Cells
Figure 2: GFP expression of T-cells cells transfected by PM-LNP-0020, checked by FACS 24 hours post transfection. Gated by non-transfected T-cells cells under identical culture conditions.
Figure 2: GFP expression of T-cells cells transfected by PM-LNP-0020, checked by FACS 24 hours post transfection. Gated by non-transfected T-cells cells under identical culture conditions.
For Research Use Only. Not for use in diagnostic procedures.