COVID-19 Spike Protein (Alpha Variant) mRNA-LNP

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Description

This product utilizes the lipid nanoparticle (LNP) technology platform for The spike protein of COVID-19 is a glycoprotein that helps the virus attach to various surfaces in the built environment, increasing the potential for virus transmission. The best-known vaccines we use are the spike protein vaccines delivered via mRNA. While the role of vaccination in controlling outbreaks is undeniable, the recurring emergence of new variants of Covid-19, such as the Delta and Omega variants, poses new challenges, particularly their rapid global spread.
This product is designed as a tool for the delivery and expression of full-length spike protein mRNA of COVID-19 Strain Alpha for vaccine research.
This product utilizes the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of the Alpha variant spike protein (COVID-19) mRNA to a variety of mammalian cells in vitro and in vivo. The LNP’s used are formulated with, SM-102, DSPC, cholesterol, and DMG-PEG2000 at optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. Alpha Spike protein consists of 1273 amino acids and its GenPept accession number is YP_009724390.

Product Overview

Composition

mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).

Cell Type Tested

HEK293S

Application & Handing

Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice. It is important to minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage

4°C; ice

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COVID-19 Spike Protein (Alpha Variant) Expression in HEK293s Cells

Figure 1: COVID-19 Spike Protein (Alpha variant) expression in HEK293s cells transfected by PM-LNP-0010, checked by FACS 48 hours post transfection. Cells were stained using 1° ab: R&D Systems anti-SARS-CoV-2 S1; 2° ab: Jackson ImmunoResearch Alexa Fluor 647 AffiniPure Goat Anti-Mouse IgG. Transfected cells were gated using non-transfected HEK293s cells under identical conditions. Cell morphology may be affected after transfection.

Antibody Titer