CD70-TF tag mRNA-LNP

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CD70 (Cluster of Differentiation 70) is a type II transmembrane glycoprotein encoded by the CD70 gene and belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27 and a surface antigen on activated but not resting T and B lymphocytes. CD70 expression is upregulated after dendritic cell maturation. CD27 is the sole receptor for CD70, a member of the TNF receptor family that is constitutively expressed on thymocytes, naive T cells, B cells, and natural killer cells. Through binding to CD27, CD70 induces the proliferation of co-stimulatory T cells, enhances the generation of cytolytic T cells, and promotes T cell activation. This cytokine has also been reported to play a role in the regulation of B cell activation, the cytotoxic function of natural killer cells, and immunoglobulin synthesis. CD70 has been shown to be overexpressed in several types of cancer, including Hodgkin's and non-Hodgkin's lymphomas, as well as certain types of solid tumors. This overexpression is associated with cancer initiation and progression, suggesting that anti-CD70 antibodies may be a possible treatment for CD70-positive lymphomas. This product is designed as a tool for the delivery and expression of TF-tagged human CD70 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hCD70-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The hCD70-TF protein is about 23 kD, consisting of hCD70 and a C-terminal TF tag (15 amino acids). The GenPept accession number for hCD70 (193 amino acids) is NP_001243.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.