CCL2 mRNA-LNP
CCL2 (Chemokine (C-C motif) ligand 2), commonly known as monocyte chemoattractant protein-1 (MCP-1), belongs to a family of secreted proteins involved in immune regulatory and inflammatory processes. Mature wild-type CCL2 protein has a full length of 76 amino acids, and its N-terminal residues are crucial in activating the CCL2 signaling pathway. The CCL2 mutant (9-76 amino acids), also known as 7ND, is a deletion of the first 8 residues of wild-type CCL2, which can bind to the C-C chemokine receptors CCR2 and CCR4 on the surface of monocytes, but cannot activate the down-regulated pathway. Therefore, the CCL2 mutant can be used as an inhibitory form of wild-type CCL2 to block the C-C chemokine receptors of CCL2 and inhibit unwanted monocyte/macrophage recruitment. This product is designed as a tool for the delivery and expression of the mature wild-type CCL2 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of CCL2 mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The GenPept accession number for CCL2 is NP_002973.
![Figure 1. ELISA using supernatants of HEK293 cells transfected with CCL-2 RNA-LNP showed secreted high level of CCL-2 cytokine 16 hours after transfection.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/PMC_2042.png?v=1680113157)
![Figure 2: The CCL2-CCR2 signal transduction pathway. When the G-protein coupled receptor, CCR2, binds to its ligand, CCL2, a series of downstream pathways are activated leading to tumor proliferation, cell growth, and increased production of CCL2](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/Pathway.png?v=1680113157)