Cas-9 Dead mRNA-LNP

Item Information
Catalog #

Cas9 (CRISPR-associated protein 9) is a dual RNA-guided DNA endonuclease associated with the clustered regularly interspaced short palindromic repeat (CRISPR) adaptive immune system originally discovered in Streptococcus pyogenes. The Cas9 protein is widely used as a genome engineering tool together with a small guide RNA (sgRNA) or gRNA to induce site-directed double-strand breaks or insertions in DNA, so-called CRISPR/Cas9 gene editing. CRISPR/Cas9 has been the basis for the development of numerous molecular systems used in research and, increasingly, in medical practice. Point mutations D10A and H840A alter 2 residues important for endonuclease activity of Cas9, resulting in Cas9 inactivation. The mutated Cas9 protein devoid of nucleolytic activity, known as Cas9 dead or dCas9, provide a technology for precise epigenetic DNA modification by delivering functional methylases and acetylases to genomic sites.
This product is designed as a tool for the delivery and expression of Cas9-dead mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of Cas9-dead mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. Cas9-dead protein is 1368 amino acids, approximately 150 kD, and the GenPept accession number is QPF47903.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.