C-myc mRNA-LNP

Item Information
Catalog #

Myc is a family of regulatory genes and proto-oncogenes encoding transcription factors. The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL) and n-myc (MYCN). c-myc (sometimes also called MYC) was the first gene identified in this family because of its homology to the viral gene v-myc. The transcription factor C-myc is a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation. C-myc regulates the transcription of specific target genes by forming heterodimers with the related transcription factor MAX and binding the E-box DNA consensus sequence. In cancer, c-myc is often constitutively expressed, leading to increased expression of many genes, some of which are involved in cell proliferation and contribute to cancer development. There is evidence that C-myc translation can be initiated from upstream out-of-frame AUG (CUG) and downstream AUG initiation sites, resulting in two isoforms with distinct N-termini. This product is designed as a tool for the delivery and expression of C-myc mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of C-myc mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The C-Myc protein is about 50 kD and consists of 454 amino acids. The GenPept accession number is NP_002458.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.