anti-HBV CAR mRNA-LNP
Hepatitis B virus (HBV) is a partially double-stranded DNA virus, a member of the Hepadnavirus family, which causes the disease hepatitis B. The virus particle consists of an outer lipid envelope and an icosahedral nucleocapsid core composed of protein. The nucleocapsid encloses the viral DNA and a DNA polymerase that has reverse transcriptase activity similar to retroviruses. The outer envelope contains embedded proteins which are involved in viral binding of, and entry into, susceptible cells.
Chronic HBV infection is a major health burden, estimated to affect approximately 290 million people worldwide. Serological HBs antigen is a classic marker of HBV infection and is usually detected by blood tests. The major surface antigen is the large S protein of the virus. Antibodies against HBs antigen are commonly regarded as neutralizing, and the presence of anti-HBs indicates resolution of HBV infection. Previous studies reported a rare serological pattern of HBsAg coexisting with anti-HBs in chronic HBV patients. The proportion of anti-HBs patients was less than 10%. In patients with chronic HBV, the coexistence of HBsAg and anti-HBs may increase the risk of hepatocellular carcinoma, which may be associated with disease progression and long-term prognosis.
This product is designed as a tool for the delivery and expression of anti-HBV CAR mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of anti-HBV CAR mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The anti-HBV CAR fusion protein in this product is approximately 58 kD and consists of anti-HBV scFv (single-chain variable fragment) linked to a second-generation CAR (chimeric antigen receptor) containing the CD8 hinge and transmembrane domain and 4-1BB and CD3ζ signaling domains. The full-length amino acid sequence of this anti-HBV CAR fusion protein is available upon request.