Anti-EPCAM/CD3 bispecific mRNA-LNP

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Description

EpCAM (epithelial cell adhesion molecule, or CD326) is a transmembrane glycoprotein that mediates Ca2+-independent homotypic cell-cell adhesion in epithelial cells. EpCAM is overexpressed in many cancers and is also expressed in cancer stem cells, making EpCAM an attractive target for immunotherapy. EpCAM is expressed on the basolateral membrane of all epithelial cells, except normal squamous stratified epithelium that is EpCAM negative. EpCAM is cleaved into an extracellular domain (EpEX) and an intracellular domain (EpICD). EpICD forms a complex with other proteins in the nucleus and promotes the transcription of various genes, including the oncogene c-myc. This has been linked to promoting tumor growth. EpEX can stimulate the cleavage of additional EpCAM molecules, creating a positive feedback loop. CD3 (cluster of differentiation 3) is a protein complex that serves as a surface marker found on all T lymphocytes. Anti-EpCAM/CD3 bispecific antibodies provide a tool to identify EpCAM as a therapeutic target. This product is designed as a tool for the delivery and expression of anti-EPCAM/CD3 bsAb mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of anti-EPCAM/CD3 bsAb mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The full-length amino acid sequence of this anti-EPCAM/CD3 bsAb mRNA-LNP product is available upon request.

Product Overview

Composition

mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).

Cell Type Tested

HEK293S

Application & Handing

Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

Storage

4°C; ice

Product Image

FACS Data for EpCAM/CD3 Bi-Specific Antibody Secreted by HEK293s Cells

Figure 1: Supernatant was collected 96 hours after transfection by PM-LNP-0018. T-cells (CD3+), LOVO cells (EpCAM+), and COLO-741 (CD3-, EpCAM-) were resuspended in the collected supernant. 1° ab: PM-LNP-0018 supernatant. 2° ab: Jackson ImmunoResearch Alexa Fluor® 647 AffiniPure Goat Anti-Human IgG (H+L). Controls used for gating were stained with non-transfected HEK293s supernatant, and the identical 2° ab.

For Research Use Only. Not for use in diagnostic procedures.