Human Anti-CS1 CAR-TF tag mRNA-LNP

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CS1 (CD2 subset-1), also known as CD319 (SLAMF7), is a human natural killer (NK) cell receptor encoded by the SLAMF7 gene. This surface antigen is a robust marker of normal and malignant plasma cells in multiple myeloma. Human NK cells express two splice variants of CS1: CS1-S lacks an intracellular domain for activation, while CS1-L contains an intracellular domain and is therefore capable of activating NK cytotoxicity. CS1 is a homophilic receptor, and CS1-CS1 interaction leads to the activation of natural cytotoxicity of NK cells. Both isoforms of CS1 are membrane bound and expressed in NK cells. However, only the CS1-L isoform is expressed in B cells and regulates B cell proliferation and autocrine.
This product is designed as a tool for the delivery and expression of human anti-CS1-TF CAR mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of human anti-CS1-TF CAR mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The human anti-CS1-TF CAR in this product is approximately 58 kD, consisting of human anti-CS1 scFv (single-chain variable fragment) linked to a second-generation CAR (chimeric antigen receptor) containing the CD8 hinge and transmembrane domain and 4-1BB and CD3ζ signaling domains. It contains an additional C-terminal TF tag (15 amino acids). The full-length amino acid sequence of this human anti-CS1-TF CAR mRNA-LNP product is available upon request.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.