STAT1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Product Overview
Entrez GenelD
6772
Aliases
ISGF-3; STAT91; DKFZp686B04100; STAT1
Host / Isotype
Rabbit / IgG
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthesized non-phosphopeptide derived from human STAT1 around the phosphorylation site of serine 727.
Formulation
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J Smooth Muscle Res. 2009 Dec;45(6):279-88.
2. Clin Exp Immunol. 2010 Jun;160(3):380-5.
2. Clin Exp Immunol. 2010 Jun;160(3):380-5.
Product Image
Western Blot
Figure 1: Western blot analysis using STAT1 Rabbit pAb against Jurkat (1), Hela (2), K562 (3) and NIH/3T3 (4) cell lysate.
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of Hela cells using STAT1 Rabbit pAb (Red). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded submaxillary gland tumor (left), colon cancer tissues (right) using STAT1 Rabbit pAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.