SOX9 Primary Antibody

Item Information
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Description

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Product Overview
Entrez GenelD
6662
Aliases
CMD1; SRA1; CMPD1; SOX9
Host / Isotype
Rabbit / IgG
Species Reactivity
Human, Mouse
Immunogen
Synthesized peptide derived from internal of human SOX9.
Formulation
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J Bone Miner Res. 2009 Dec;24(12):2039-49.
2. J Med Genet. 2009 Oct;46(10):649-56.
Product Image
Western Blot
Figure 1: Western blot analysis using SOX9 Rabbit pAb against K562 (1), HCT116 (2), SW620 (3), LOVO (4), NTERA-2 (5), HEK293 (6) and HepG2 (7) cell lysate.
Figure 1: Western blot analysis using SOX9 Rabbit pAb against K562 (1), HCT116 (2), SW620 (3), LOVO (4), NTERA-2 (5), HEK293 (6) and HepG2 (7) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SOX9 Rabbit pAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SOX9 Rabbit pAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of MCF-7 cells using SOX9 Rabbit pAb (red). Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of MCF-7 cells using SOX9 Rabbit pAb (red). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.