PCNA Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Product Overview
Entrez GenelD
5111
Aliases
MGC8367; PCNA
Host / Isotype
Rabbit / IgG
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from internal of human PCNA.
Formulation
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Cell. 2010 Jan 8;140(1):123-35.
2. Cancer Invest. 2010 Jul;28(6):635-41.
2. Cancer Invest. 2010 Jul;28(6):635-41.
Product Image
Western Blot
Figure 1: Western blot analysis using PCNA Rabbit pAb against HepG2 (1) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer (left), esophagus cancer tissues (right) using PCNA Rabbit pAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.