PKHD1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesFPC; Tigm1; AI118496; AI182499
ProductOrderP
Clone#8G12A1
Entrez GenelD241035
FormulationPurified antibody in PBS with 0.05% sodium azide
HostRat
IsotypeIgG1
ImmunogenPurified recombinant fragment of mouse PKHD1 (AA: 3878-4060) expressed in E. Coli.
MW445kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
3878-4060
Catalog#: 30740
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Images
Western Blot
Figure 1: Western blot analysis using PKHD1 mAb against mouse PKHD1(AA: 3878-4060) recombinant protein. (Expected MW is 23 kDa)
Figure 1: Western blot analysis using PKHD1 mAb against mouse PKHD1(AA: 3878-4060) recombinant protein. (Expected MW is 23 kDa)
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2:Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionMutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, the lack of specific antibodies to the mouse protein impairs the study of splicing, post-translational processing, shedding, and temporal and spatial expression of endogenous fibrocystin at the cellular and subcellular level.
References (references)
References (references)J Am Soc Nephrol. 2011 Dec;22(12):2266-77.
Am J Pathol. 2008 Feb;172(2):417-29.