PKHD1 Primary Antibody
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Description
Mutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, the lack of specific antibodies to the mouse protein impairs the study of splicing, post-translational processing, shedding, and temporal and spatial expression of endogenous fibrocystin at the cellular and subcellular level.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using PKHD1 mAb against mouse PKHD1(AA: 3878-4060) recombinant protein. (Expected MW is 23 kDa)
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.