phospho-NLRC4(Ser-533) Primary Antibody

Item Information

Catalog #

Size

Price

Description

NLRC4 is a cytosolic NOD (nucleotide binding and oligomerization domain)-like receptor (NLR) that can trigger inflammasome formation in response to bacterial flagellin, an immunodominant antigen in the intestine.

Product Overview

Entrez GenelD

268973

Aliases

CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik

Clone#

4B7B7

Host / Isotype

Rat / IgG2b

Species Reactivity

Human, Mouse

Immunogen

Synthesized peptide of mouse phospho-NLRC4(Ser-533) (AA: 525-538) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1.Mucosal Immunol. 2014 Jul;7(4):775-85.
2.Mucosal Immunol. 2012 May;5(3):288-98.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using phospho-NLRC4(Ser-533) rat mAb against NIH/3T3 (1) cell lysate.

Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.