ZAP70 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Product Overview
Entrez GenelD
7535
Aliases
SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Clone#
1F3G9
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human ZAP70 (AA: 169-390) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.FASEB J. 2018 Sep;32(9):4824-4835. 2.Haematologica. 2017 Feb;102(2):346-355.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ZAP70 mAb against human ZAP70 (AA: 169-390) recombinant protein. (Expected MW is 27.9 kDa)
Western Blot
Figure 3:Western blot analysis using ZAP70 mAb against HEK293 (1) and ZAP70 (AA: 169-390)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ZAP70 mouse mAb against MLOT4 (1) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using ZAP70 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.