ZAP70 Primary Antibody
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Purified antibody in PBS with 0.05% sodium azide
4?; -20? for long term storage
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ZAP70 mAb against human ZAP70 (AA: 169-390) recombinant protein. (Expected MW is 27.9 kDa)
Figure 3:Western blot analysis using ZAP70 mAb against HEK293 (1) and ZAP70 (AA: 169-390)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ZAP70 mouse mAb against MOLT4 (1) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.