XRCC6 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Product Overview

Entrez GenelD

2547

Aliases

ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Clone#

7A9E7

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human XRCC6 (AA: 6-214) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Clin Cancer Res. 2013 Mar 15;19(6):1547-56.
2. Mol Carcinog. 2012 Oct;51 Suppl 1:E183-90.

Product Image

Western Blot

Figure 1: Western blot analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)

Western Blot

Figure 2: Western blot analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using XRCC6 mouse mAb against PC-2 (1), A549 (2), A431 (3), HepG2 (4), K562 (5) cell lysate.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric