Mouse Monoclonal Antibody to XRCC1
Item Information
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Size
Price
Description
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Product Overview
Entrez GenelD
7515
Aliases
RCC; SCAR26
Clone#
4D10D3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human XRCC1 (AA: 1-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,DNA Repair (Amst). 2020 Sep;93:102917.2,Zhonghua Yi Xue Za Zhi. 2021 Mar 23;101(11):759-765.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c1df9001baea4831966eeb69c147e52a950fa7785779ae9de94e2d908524376e.jpg?v=1666705906)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using XRCC1 mAb against human XRCC1 (AA: 1-150) recombinant protein. (Expected MW is 42.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3934b5659383473f6f0879237e9b5ebc2d06279c7cb6d3ca370e27221cbd9a16.jpg?v=1666705906)
Figure 2:Western blot analysis using XRCC1 mAb against human XRCC1 (AA: 1-150) recombinant protein. (Expected MW is 42.5 kDa)
Western Blot
![Figure 3:Western blot analysis using XRCC1 mAb against HEK293-6e (1) and human XRCC1-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e403da15dbc36e9b2013803230bca5a52cbef8bb63bcccbf908b4401113d0355.jpg?v=1666705906)
Figure 3:Western blot analysis using XRCC1 mAb against HEK293-6e (1) and human XRCC1-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of A375 cells using XRCC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/923079f213b506c035ce777e5ddf6f8714c8b6bf5329f9da07d99f6aad7a0921.jpg?v=1666705906)
Figure 4:Flow cytometric analysis of A375 cells using XRCC1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using XRCC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5b1e96dd58e5e08b70c585af29fd95a2c4cf6d021683d7b017ac573f422789e1.jpg?v=1666705906)
Figure 5:Flow cytometric analysis of Jurkat cells using XRCC1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of K562 cells using XRCC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f5385c46ba301bfc5593a5ab9c8c3f8a11bc0db6fdda57f824032a6c6c1cd6b1.jpg?v=1666705906)
Figure 6:Flow cytometric analysis of K562 cells using XRCC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using XRCC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4e3059723d1270450b2616e833030d4d333755f71afdc7fda32f3062dd1f5880.jpg?v=1666705906)
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using XRCC1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using XRCC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5b6dc6503d50684c517b5a8171e83057f944e9eace9a75b1e9098bc242ef0be7.jpg?v=1666705906)
Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using XRCC1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using XRCC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5974f8d485ef6c056dc1a73c559c55816bd70a4004d079759db3aeb08336fa57.jpg?v=1666705906)
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using XRCC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.