XPC Primary Antibody
Item Information
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Price
Description
The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.
Product Overview
Entrez GenelD
7508
Aliases
XP3; RAD4; XPCC; p125
Clone#
5F4B11
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human XPC (AA: 32-133) expressed in mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Acta Medica (Hradec Kralove). 2020;63(3):101-112.
2,Nat Commun. 2020 Nov 17;11(1):5834.
2,Nat Commun. 2020 Nov 17;11(1):5834.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using XPC mAb against human XPC (AA: 32-133) recombinant protein. (Expected MW is 41.9 kDa)
Western Blot
Figure 3:Western blot analysis using XPC mouse mAb against SW480(1) ,A431(2) ,T47D(3) ,HT-29(4) ,A549 (5)and C2C12(6) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Hela cells using XPC mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.